Role of somite patterning in the formation of Weberian apparatus and pleural rib in zebrafish

Kagari Akama, Kanami Ebata, Akiteru Maeno, Tomohito Taminato, Shiori Otosaka, Keiko Gengyo-Ando, Junichi Nakai, Kyo Yamasu, Akinori Kawamura

Research output: Contribution to journalArticlepeer-review

Abstract

In the vertebrate body, a metameric structure is present along the anterior–posterior axis. Zebrafish tbx6−/− larvae, in which somite boundaries do not form during embryogenesis, were shown to exhibit abnormal skeletal morphology such as rib, neural arch and hemal arch. In this study, we investigated the role of somite patterning in the formation of anterior vertebrae and ribs in more detail. Using three-dimensional computed tomography scans, we found that anterior vertebrae including the Weberian apparatus were severely affected in tbx6−/− larvae. In addition, pleural ribs of tbx6 mutants exhibited severe defects in the initial ossification, extension of ossification, and formation of parapophyses. Two-colour staining revealed that bifurcation of ribs was caused by fusion or branching of ribs in tbx6−/−. The parapophyses in tbx6−/− juvenile fish showed irregular positioning to centra and abnormal attachment to ribs. Furthermore, we found that the ossification of the distal portion of ribs proceeded along myotome boundaries even in irregularly positioned myotome boundaries. These results provide evidence of the contribution of somite patterning to the formation of the Weberian apparatus and rib in zebrafish.

Original languageEnglish
Pages (from-to)622-629
Number of pages8
JournalJournal of Anatomy
Volume236
Issue number4
DOIs
Publication statusPublished - 2020 Apr 1

Keywords

  • Weberian apparatus
  • myotome
  • pleural rib
  • somite
  • zebrafish

ASJC Scopus subject areas

  • Anatomy
  • Ecology, Evolution, Behavior and Systematics
  • Histology
  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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