Role of Rab27 in synaptic transmission at the squid giant synapse

Eunah Yu, Eiko Kanno, Soonwook Choi, Mutsuyuki Sugimori, Jorge E. Moreira, Rodolfo R. Llinás, Mitsunori Fukuda

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Small GTPase Rab is a member of a large family of Ras-related proteins, highly conserved in eukaryotic cells, and thought to regulate specific type(s) and/or specific step(s) in intracellular membrane trafficking. Given our interest in synaptic transmission, we addressed the possibility that Rab27 (a close isoform of Rab3) could be involved in cytosolic synaptic vesicle mobilization. Indeed, preterminal injection of a specific antibody against squid Rab27 (anti-sqRab27 antibody) combined with confocal microscopy demonstrated that Rab27 is present on squid synaptic vesicles. Electrophysiological study of injected synapses showed that the anti-sqRab27 antibody inhibited synaptic release in a stimulation-dependent manner without affecting presynaptic action potentials or inward Ca2+ current. This result was confirmed in in vitro synaptosomes by using total internal reflection fluorescence microscopy. Thus, synaptosomal Ca2+-stimulated release of FM1-43 dye was greatly impaired by intraterminal anti-sqRab27 antibody. Ultrastructural analysis of the injected giant preterminal further showed a reduced number of docked synaptic vesicles and an increase in nondocked vesicular profiles distant from the active zone. These results, taken together, indicate that Rab27 is primarily involved in the maturation of recycled vesicles and/or their transport to the presynaptic active zone in the squid giant synapse.

Original languageEnglish
Pages (from-to)16003-16008
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number41
DOIs
Publication statusPublished - 2008 Oct 14

Keywords

  • Griscelli syndrome
  • Rabphilin
  • Small GTPase
  • Total internal reflection fluorescence microscopy
  • Vesicle exocytosis

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Role of Rab27 in synaptic transmission at the squid giant synapse'. Together they form a unique fingerprint.

  • Cite this