Role of Porins in the Antibiotic Susceptibility of Pseudomonas aeruginosa: Construction of Mutants with Deletions in the Multiple Porin Genes

Hiroshi Yoneyama, Yoshinori Yamano, Taiji Nakae

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

We inserted deletions in the chromosomal genes of Pseudomonas aeruginosa coded for the outer membrane porins, proteins C, D2, or E1, and all possible combinations of these proteins by the gene replacement technique and selecting for imipenem-resistance. Determination of the minimum inhibitory concentrations of β-lactams, fluoroquinolones, chloramphenicol and gentamicin in these mutants revealed that most mutants showed equal susceptibility to the porin-sufficient strain. The only exception was that imipenem and meropenem showed increased minimum inhibitory concentrations in all of the mutants lacking protein D2. These results firmly established that the P. aeruginosa porins identified so far form the pores do not accommodate the passage of most antipseudomonal antibiotics, with the exception of carbapenems.

Original languageEnglish
Pages (from-to)88-95
Number of pages8
JournalBiochemical and biophysical research communications
Volume213
Issue number1
DOIs
Publication statusPublished - 1995 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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