Role of polar injection solvent in ion-pair reversed-phase partition high performance liquid chromatography

Emiko Kaneko, Hitoshi Hoshino, Takao Yotsuyanagi

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The role of polar solvents in the injection samples in ion-pair reversed-phase partition high performance liquid chromatography has been investigated by using metal chelates with 2,2′-dihydroxyazobenzene and 4-(2-pyridylazo)resorcinol. When the chelates were separated on C18-bonded silica packing with an aqueous methanol mobile phase containing tetrabutylammonium bromide and detected spectrophotometrically, the peak profile was significantly distorted by the organic solvent in the sample solution. When a solvent having a polarity greater than the mobile phase was used for injection, the peaks were better resolved and sharpened. The best peak profile, assessed by peak height and resolution, was obtained with aqueous sample injection. An interpretation of this phenomenon is given based on the concentration process of analytes at the top of the column.

Original languageEnglish
Pages (from-to)127-134
Number of pages8
JournalSolvent Extraction Research and Development
Volume1998
Issue number5
Publication statusPublished - 1998 Dec 1

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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