Role of platelet-activating-factor (PAF) on cellular responses after stimulation with leptospire lipopolysaccharide

Emiko Isogai, Kimiharu Hirose, Koichi Kimura, Shunji Hayashi, Toru Kubota, Nobuhiro Fujii, Hiroshi Isogai

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Leptospire lipopolysaccharide (LPS) stimulated the adherence of polymorphonuclear neutrophils (PMNs) to human umbilical vein endothelial cells (HUVEC). Enhanced PMN adherence in response to leptospire LPS can be mediated by platelet-activator-factor (PAF), because a PAF antagonist reduced adherence. Leptospire LPS also induced the adherence platelets or U937. The second experiment involved leptospire LPS elicited platelet aggregation in a PMN-platelet mixture, because leptospire LPS stimulated human PMN but not the human platelets. The platelet response was observed only in the mixture system and was inhibited by a PAF antagonist. PAF could be an important pathogenic factor in human leptospirosis.

Original languageEnglish
Pages (from-to)271-275
Number of pages5
JournalMICROBIOLOGY and IMMUNOLOGY
Volume41
Issue number3
DOIs
Publication statusPublished - 1997

Keywords

  • Human umbilical vein endothelial cells
  • Leptospire lipopolysaccharide
  • Platelet-activating-factor
  • Polymorphonuclear neutrophils

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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