Role of peroxisome proliferator-activated receptor β/δ in chicken adipogenesis

Kan Sato, Takashi Yonemura, Hiroshi Ishii, Masaaki Toyomizu, Toshihiko Kamada, Yukio Akiba

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)


The adipocyte differentiation process involves a cascade of transcriptional events that culminates in the expression of peroxisome proliferator-activated receptor (PPAR)γ. The present study was undertaken to identify the role of PPARβ/δ in chicken adipocyte differentiation, with experiments performed using a PPARγ agonist, a PPARβ/δ agonist, a PPARγ antagonist and fatty acid (oleate). Preadipocyte cells cultured in a differentiation medium (DMEM containing 500 nM dexamethasone, 0.5 mM 3-isobutyl-1-methylxanthine, 20 μg/mL bovine insulin and 10% fetal bovine serum) supplemented with 200 μM oleate resulted in a significant increase in adipocyte fatty acid binding protein (aP2) mRNA expression after 24 h and 7 d of culture compared to cells cultured in a differentiation medium alone, while supplementation of the differentiation medium with GW501516 (a PPARβ/δ agonist) did not affect aP2 mRNA expression levels. Supplementation of the differentiation medium with troglitazone (a PPARγ agonist) and GW501516 induced preadipocyte differentiation; a significant increase of aP2 mRNA expression was observed in cells after incubation for 7 d, but not after 24 h of incubation. These results suggest that PPARβ/δ does not play a key role in adipocyte differentiation, but it does enhance the transformation of immature into mature adipocytes in chickens. In addition, oleate functions not only as an activator of PPARs but also induces PPARγ gene expression via alternative pathway of PPARs activation. These results establish the importance of exogenous fatty acid in the processes of adipogenesis and fat accumulation in chickens.

Original languageEnglish
Pages (from-to)370-375
Number of pages6
JournalComparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Issue number3
Publication statusPublished - 2009 Nov
Externally publishedYes


  • Adipogenesis
  • Chicken
  • Glycerol-3-phosphate dehydrogenase
  • PPARβ/δ
  • PPARγ
  • aP2

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Aquatic Science
  • Animal Science and Zoology
  • Molecular Biology


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