Role of OsNPR1 in rice defense program as revealed by genome-wide expression analysis

Shoji Sugano, Chang Jie Jiang, Shin Ichi Miyazawa, Chisato Masumoto, Katsumi Yazawa, Nagao Hayashi, Masaki Shimono, Akira Nakayama, Mitsue Miyao, Hiroshi Takatsuji

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

NPR1 is a central regulator of salicylic-acid (SA)-mediated defense signaling in Arabidopsis. Here, we report the characterization of OsNPR1, an Oryzae sativa (rice) ortholog of NPR1, focusing on its role in blast disease resistance and identification of OsNPR1-regulated genes. Blast resistance tests using OsNPR1 knockdown and overexpressing rice lines demonstrated the essential role of OsNPR1 in benzothiadiazole (BTH)-induced blast resistance. Genome-wide transcript profiling using OsNPR1-knockdown lines revealed that 358 genes out of 1,228 BTH-upregulated genes and 724 genes out of 1,069 BTH-downregulated genes were OsNPR1-dependent with respect to BTH responsiveness, thereby indicating that OsNPR1 plays a more vital role in gene downregulation. The OsNPR1-dependently downregulated genes included many of those involved in photosynthesis and in chloroplast translation and transcription. Reduction of photosynthetic activity after BTH treatment and its negation by OsNPR1 knockdown were indeed reflected in the changes in Fv/Fm values in leaves. These results imply the role of OsNPR1 in the reallocation of energy and resources during defense responses. We also examined the OsNPR1-dependence of SA-mediated suppression of ABA-induced genes.

Original languageEnglish
Pages (from-to)549-562
Number of pages14
JournalPlant Molecular Biology
Volume74
Issue number6
DOIs
Publication statusPublished - 2010 Dec
Externally publishedYes

Keywords

  • Abscisic acid
  • Benzothiadiazole
  • Blast resistance
  • NPR1
  • OsNPR1
  • Photosynthesis

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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  • Cite this

    Sugano, S., Jiang, C. J., Miyazawa, S. I., Masumoto, C., Yazawa, K., Hayashi, N., Shimono, M., Nakayama, A., Miyao, M., & Takatsuji, H. (2010). Role of OsNPR1 in rice defense program as revealed by genome-wide expression analysis. Plant Molecular Biology, 74(6), 549-562. https://doi.org/10.1007/s11103-010-9695-3