TY - JOUR
T1 - Role of ompD2 and chromosomal β-lactamase in carbapenem resistance in clinical isolates of pseudomonas aeruginosa
AU - Satake, S.
AU - Yoneyama, H.
AU - Nakae, T.
N1 - Funding Information:
This study was supported by grants from the Ministry of Education of Japan, Ohyama Health Foundation Inc., and the Organization of General Research of Tokai University. The clinical isolates were kind gift of K. Okuzumi of the University of Tokyo, School of Medicine. Thanks are due to the pharmaceutical companies who kindly provided the reagent-grade antibiotics. Strain PAO4098 was a gift of Dr H. Matsumoto of Shinsyu University, School of Medicine.
PY - 1991/8
Y1 - 1991/8
N2 - Imipenem-resistant clinical isolata of Pseudomonas aeruginaso were divided into two categories: (i) isolates that were moderately resistant to imipenem (MIC 6·25 mg/L) that produced trace amounts of protein D2 detected with immunoblotting using anti-protein D2 antibody, but not when stained with Coomassie blue and had inducible class 1 β-lactamase expression; (ii) isolates that were highly resistant to several β-lactamase, including meropenem, with no protein D2 by staining or immunoblotting and had stably derepressed β-lactamase. Laboratory strains were isolated and analyzed: (i) mutants lacking protein D2, or (ii) Lacking protein D2 and producing stably derepressed β-lactamase with carbapenem resistance similar to the clinical isolates. (iii) mutants producing undetectable β-lactamase which were four-fold more susceptible to imipenem than the mutant producing stably derepressed β-lactamase or the strain with inducible β-lactamase. These data suggests that β-lactamase and outer membrane permeability govern meropenem-resistance in P. aeruginosa
AB - Imipenem-resistant clinical isolata of Pseudomonas aeruginaso were divided into two categories: (i) isolates that were moderately resistant to imipenem (MIC 6·25 mg/L) that produced trace amounts of protein D2 detected with immunoblotting using anti-protein D2 antibody, but not when stained with Coomassie blue and had inducible class 1 β-lactamase expression; (ii) isolates that were highly resistant to several β-lactamase, including meropenem, with no protein D2 by staining or immunoblotting and had stably derepressed β-lactamase. Laboratory strains were isolated and analyzed: (i) mutants lacking protein D2, or (ii) Lacking protein D2 and producing stably derepressed β-lactamase with carbapenem resistance similar to the clinical isolates. (iii) mutants producing undetectable β-lactamase which were four-fold more susceptible to imipenem than the mutant producing stably derepressed β-lactamase or the strain with inducible β-lactamase. These data suggests that β-lactamase and outer membrane permeability govern meropenem-resistance in P. aeruginosa
UR - http://www.scopus.com/inward/record.url?scp=0025799683&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025799683&partnerID=8YFLogxK
U2 - 10.1093/jac/28.2.199
DO - 10.1093/jac/28.2.199
M3 - Article
C2 - 1778851
AN - SCOPUS:0025799683
VL - 28
SP - 199
EP - 207
JO - Journal of Antimicrobial Chemotherapy
JF - Journal of Antimicrobial Chemotherapy
SN - 0305-7453
IS - 2
ER -