Role of Nup98 in nuclear entry of human immunodeficiency virus type 1 cDNA

Hirotaka Ebina, Jun Aoki, Shunsuke Hatta, Takeshi Yoshida, Yoshio Koyanagi

Research output: Contribution to journalArticlepeer-review

48 Citations (Scopus)

Abstract

Human immunodeficiency virus type 1 (HIV-1), like other lentiviruses, can infect non-dividing cells. The lentiviruses are most likely to have evolved a nuclear import strategy to import HIV-1 cDNA and viral protein complex through the nuclear pore complex (NPC) formed by nucleoporin proteins (Nup). In this study, we found that synthesis of integrated and 2LTR but not full-length form of HIV-1 cDNA was clearly impaired in culture via transduction of vesicular stomatitis virus matrix protein (VSV M), an inhibitor protein, through binding to the phenylalanine-glycine (FG) repeat region of Nup98. The impairment of synthesis of integrated and 2LTR DNA with VSV M was restored by ectopic overexpression of Nup98. A series of experiments using Nup98-depleted NPC by the small interfering RNA (siRNA) technique showed specific impairment of NPC structure and some functions, including nuclear import of HIV-1 cDNA. Our results suggest that Nup98 on the NPC specifically participates in the nuclear entry of HIV-1 cDNA following HIV-1 entry.

Original languageEnglish
Pages (from-to)715-724
Number of pages10
JournalMicrobes and Infection
Volume6
Issue number8
DOIs
Publication statusPublished - 2004 Jul

Keywords

  • HIV-1
  • NPC
  • Nuclear import
  • Nucleoporin

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Infectious Diseases

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