Role of nitric oxide in the pancreatic blood flow response to caerulein

Akihiko Satoh, Tooru Shimosegawa, Takashi Abe, Yoshifumi Kikuchi, Reishi Abe, Masaru Koizumi, Takayoshi Toyota

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

To clarify the role of nitric oxide (NO) in the pancreas, blood flow in the rat pancreas (pancreatic blood flow: PBF) was investigated by the hydrogen clearance technique using a specific NO synthase inhibitor, Nw-nitro-L-arginine (L-NNA). Continuous infusion of caerulein at doses of 5 and 20 μg/kg/h caused a significant increase in PBF in the early phase of caerulein infusion. The caerulein-induced increase in PBF was not affected significantly by atropine sulfate (100 μg/kg), nor by phe-noxybenzamine (5 mg/kg) plus propranolol (50 μg/kg). Administration of L-NNA (0.5, 5, or 30 mg/kg) did not affect the basal PBF, but at 5 mg/kg it inhibited completely the caerulein-induced increase in PBF. The inhibitory action of L-NNA was reversed by a large dose of L-arginine (100 mg/kg bolus, i.v., followed by a continuous infusion at 400 mg/kg/h), but not by its enantiomer D-arginine. These results strongly suggest that NO has a mediator role in the early phase vascular response of the pancreas to superphysiologic doses of caerulein.

Original languageEnglish
Pages (from-to)574-579
Number of pages6
JournalPancreas
Volume9
Issue number5
DOIs
Publication statusPublished - 1994 Sep

Keywords

  • Blood flow
  • Caerulein
  • NO
  • Pancreas

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

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