Abstract
To evaluate the role of Na+-glucose cotransport in fluid absorption across alveolar epithelial walls in isolated rat lungs, we measured the inhibitory effect of amiloride (a Na+ channel blocker) and phlorizin (a Na+-glucose cotransport blocker) on the fluid absorption rate in fluid- filled lungs. Amiloride (10-5 - 10-4 M) reduced alveolar fluid absorption by 30%. This value was similar to that obtained in the presence of 10-3 M phlorizin. The coefficient of Na+-glucose cotransport was estimated to be 2.5. The strong correlation between Na+ escape and fluid absorption (r = 0.907) was not affected by phlorizin. These findings suggest that the impact of Na+-glucose cotransport was similar to that of Na+ transport alone, and that glucose molecules transported by Na+-glucose do not play an important role in alveolar fluid absorption across rat alveolar epithelium.
Original language | English |
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Pages (from-to) | 1109-1114 |
Number of pages | 6 |
Journal | Japanese Journal of Thoracic Diseases |
Volume | 34 |
Issue number | 10 |
Publication status | Published - 1996 Dec 7 |
Keywords
- Alveolar epithelium
- Na -glucose cotransport
- Na channel
- Phlorizin
- Pulmonary edema
ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine