Role of Link N in modulating inflammatory conditions

Mu Chen Yang, Ding Han Wang, Juin Hong Cherng, Wan Chun Li, Po Yen Lin, Wun Eng Hsu, Ming Lun Hsu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Aims: To elucidate the role of Link N in regulating inflammatory molecules from human mesenchymal stem cells (hMSCs) under interleukin (IL)-1β stimulation in vitro and under Complete Freund's Adjuvant (CFA)-induced arthritis of the temporomandibular joint (TMJ) in vivo. Methods: In vitro analysis of inflammatory cytokines and epithelial-mesenchymal transition (EMT) genes in hMSCs treated with Link N, IL-1β, and co-stimulation of IL-1β and Link N was undertaken using Luminex multiplex assays and real-time polymerase chain reaction, respectively. To determine the impact of Link N in ameliorating TMJ tissue homeostasis in arthritic conditions, histologic changes in CFA-induced arthritic TMJ tissues followed by application of Link N were examined. All data were analyzed using one-way analysis of variance with Bonferroni post hoc test. Results: Increased levels of IL-6; interferon gamma-inducible protein-10; and regulated upon activation, normal T cell expressed, and secreted (RANTES) were detected in response to IL-1β treatment, but these levels were significantly decreased in the co-stimulation group. In contrast, secreted IL-4, IL-10, and transforming growth factor β1-β3 proteins, as well as intracellular erb-b2 receptor tyrosine kinase 3 and Nodal homolog genes, were increased significantly in the co-stimulation group compared to the IL-1β group. Histologic analysis showed significant recovery for rat condyle thickness in the Link N-treated group when compared to the CFA-induced arthritis group. Conclusion: These findings indicate that Link N could modulate inflammation and EMT in vitro and repair arthritis-mediated TMJ disruption in vivo. Link N could be a potential therapeutic agent for TMJ disorder patients.

Original languageEnglish
Pages (from-to)114-122
Number of pages9
JournalJournal of Oral and Facial Pain and Headache
Volume33
Issue number1
DOIs
Publication statusPublished - 2019 Mar 22
Externally publishedYes

Keywords

  • Arthritis
  • Complete Freund's adjuvant
  • Inflammation
  • Link N
  • Temporomandbular joint

ASJC Scopus subject areas

  • Dentistry (miscellaneous)
  • Clinical Neurology
  • Anesthesiology and Pain Medicine

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  • Cite this

    Yang, M. C., Wang, D. H., Cherng, J. H., Li, W. C., Lin, P. Y., Hsu, W. E., & Hsu, M. L. (2019). Role of Link N in modulating inflammatory conditions. Journal of Oral and Facial Pain and Headache, 33(1), 114-122. https://doi.org/10.11607/ofph.1952