Role of ghrelin in the pancreatic exocrine secretion via mitogen-activated protein kinase signaling in rats

Kyung Hoon Lee, Jae Sung Lee, Tao Wang, Jin Ju Oh, Sanggun Roh, Hong Gu Lee

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Background: This study was performed to investigate the impact of exogenous ghrelin on the pancreatic α-amylase outputs and responses of pancreatic proteins to ghrelin that may relate to pancreatic exocrine. Methods: Sprague-Dawley male rats (9 weeks old, 300 ± 10 g) were injected with ghrelin via intraperitoneal (i.p.) infusion at dosage of 0, 0.1, 1.0 and 10.0 μg/kg body weight (BW), respectively. The plasma ghrelin and cholecystokinin (CCK) level were determined using enzyme immunoassay kit; the mRNA expression of ghrelin receptor (GHSR-1α) and growth hormone (GH) receptor were assessed by reverse transcription PCR; the expressions of pancreatic α-amylase activity, extracellular-signal-regulated kinases (ERK), phosphorylated extracellular-signal-regulated kinases (pERK) and c-Jun N-terminal kinase (JNK) were evaluated by western blotting; moreover the responses of pancreatic proteins to ghrelin were analyzed using the two-dimensional gel electrophoresis system. Results: The exogenous ghrelin (1.0 and 10.0 μg/kg BW) elevated the level of plasma ghrelin (p < 0.05), and suppressed the expression of pancreatic α-amylase at a dose of 10.0 μg/kg BW (p < 0.05). No difference in the level of plasma CCK was observed, even though rats were exposed to any dose of exogenous ghrelin. In addition, a combination of western blot and proteomic analysis revealed exogenous ghrelin (10.0 μg/kg BW) induced increasing the JNK and ERK expressions (p < 0.05) and four proteins such as Destrin, Anionic trypsin-1, Trypsinogen, and especially eukaryotic translation initiation factor 3 in rat pancreas. Conclusions: Taken together, exogenous ghrelin by i.p. infusion plays a role in the pancreatic exocrine secretion via mitogen-activated protein kinase signaling pathway.

Original languageEnglish
Article number16
JournalJournal of Animal Science and Technology
Volume59
Issue number7
DOIs
Publication statusPublished - 2017 Jul 24

Keywords

  • Cholecystokinin
  • Ghrelin
  • Pancreatic exocrine
  • Sprague-Dawley rats
  • α-Amylase activity

ASJC Scopus subject areas

  • Food Science
  • Ecology
  • Animal Science and Zoology
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • veterinary (miscalleneous)

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