Role of ET(B) receptors and nitric oxide in adrenal catecholamine secretion in anesthetized dogs

Akio Hosokawa, Takahiro Nagayama, Kimiya Masada, Makoto Yoshida, Mizue Suzuki-Kusaba, Hiroaki Hisa, Tomohiko Kimura, Susumu Satoh

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

We examined the effects of sarafotoxin 6c (S6c), an endothelin-B (ET(B)) receptor agonist, on adrenal catecholamine secretion in response to cholinergic stimuli in pentobarbital sodium-anesthetized dogs. Drugs were administered intra-arterially into the adrenal gland through the phrenicoabdominal artery. Infusion of S6c attenuated increases in adrenal catecholamine output induced by splanchnic nerve stimulation. The inhibitory effect of S6c on the catecholamine secretion response was suppressed with a selective ET(B) receptor antagonist N-cis 2,6-dimethylpiperidinocarbonyl-L- γ-methylleucyl-D-1-methoxycarbonyltryptophanyl-D-norleucine (BQ-788), a nitric oxide synthase (NOS) inhibitor N(ω)-nitro-L-arginine methyl ester, and a neuronal NOS inhibitor 7-nitroindazole monosodium salt (7-NINA). Similar results were obtained with the catecholamine secretion response induced by injection of ACh. 7-NINA alone did not affect these catecholamine secretion responses. These results suggest that ET(B) receptors play an inhibitory role in adrenal catecholamine secretion by activating neuronal NOS, whereas neuronal NOS is unlikely to be involved in regulation of adrenal catecholamine secretion in the absence of simultaneous ET(B) receptor stimulation.

Original languageEnglish
Pages (from-to)R1051-R1056
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume277
Issue number4 46-4
DOIs
Publication statusPublished - 1999 Oct

Keywords

  • 7-nitroindazole monosodium salt
  • Acetylcholine
  • Neuronal nitric oxide synthase
  • Sarafotoxin 6c
  • Splanchnic nerve stimulation

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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