Role of endogenous PACAP in catecholamine secretion from the rat adrenal gland

Y. Fukushima, H. Hikichi, K. Mizukami, T. Nagayama, M. Yoshida, M. Suzuki-Kusaba, H. Hisa, T. Kimura, S. Satoh

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

We elucidated the contribution of endogenous pituitary adenylate cyclase-activating polypeptide (PACAP) to neurally evoked catecholamine secretion from the isolated perfused rat adrenal gland. Infusion of PACAP (100 nM) increased adrenal epinephrine and norepinephrine output. The PACAP-induced catecholamine output responses were inhibited by the PACAP type I receptor antagonist PACAP-(6-38) (30-3,000 nM) but were resistant to the PACAP type II receptor antagonist [Lys1,Pro2,5,Ara3,4,Tyr6]- vasoactive intestinal peptide (LPAT-VIP;30-3,000 nM). Transmural electrical stimulation (ES1-10 Hz) or infusion of ACh (6-200 nM) increased adrenal epinephrine and norepinephrine output. PACAP-(6-38) (3,000 nM), but not LPAT-VIP, also inhibited the ES-induced catecholamine output responses. However, PACAP-(6-38) did not affect the ACh-induced catecholamine output responses. PACAP at low concentrations (0.3-3 nM), which had no influence on catecholamine output, enhanced the ACh-induced catecholamine output responses, but not the ES-induced catecholamine output responses. These results suggest that PACAP is released from the nerve endings to facilitate the neurally evoked catecholamine secretion through PACAP type I receptors in the rat adrenal gland.

Original languageEnglish
Pages (from-to)R1562-R1567
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume281
Issue number5 50-5
DOIs
Publication statusPublished - 2001

Keywords

  • Acetylcholine
  • Adrenal chromaffin cells
  • Pituitary adenylate cyclase-activating polypeptide -(6-38)
  • Pituitary adenylate cyclase-activating polypeptide receptor antagonists
  • Transmural electrical stimulation

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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