TY - JOUR
T1 - Role of endogenous endothelins in catecholamine secretion in the rat adrenal gland
AU - Nagayama, Takahiro
AU - Kuwakubo, Fumiyo
AU - Matsumoto, Takayuki
AU - Fukushima, Yasuo
AU - Yoshida, Makoto
AU - Suzuki-Kusaba, Mizue
AU - Hisa, Hiroaki
AU - Matsumura, Yasuo
AU - Kimura, Tomohiko
AU - Satoh, Susumu
N1 - Funding Information:
The authors are grateful to Fujisawa Pharmaceutical (Osaka, Japan) and Banyu Pharmaceutical (Tsukuba, Japan) for their generous donation of FR139317 and BQ-788, respectively. This work was supported in part by Research Fellowships of Japan Society for the Promotion of Science for Young Scientists and by Grants No. 10877371 for Scientific Research from The Ministry of Education, Science and Culture, Japan.
PY - 2000/10/6
Y1 - 2000/10/6
N2 - We investigated the role of endogenous endothelins in catecholamine secretion in response to transmural electrical stimulation in the retrogradely perfused rat adrenal gland. (R)2-[(R)-2-[(S)-2-[[1-(hexahydro-1H-azepinyl)]carbonyl]amino-4-methyl-pentanoyl]amino-3-[3-(1-methyl-1H-indoyl)]propionyl]amino-3-(2-pyridyl) propionic acid (FR139317; 0.03-3 μM), an endothelin ET(A) receptor antagonist, inhibited the electrical stimulation-induced epinephrine and norepinephrine output. Neither N-cis-2,6-dimethylpiperidinocarbonyl-L-γ-methylleucyl-D-1-methoxycarbonyltryptophanyl-D-norleucine (BQ-788; 0.03-3 μM), an endothelin ET(B) receptor antagonist, nor phosphoramidon (1-100 mM), an endothelin-converting enzyme inhibitor, affected the catecholamine output responses. However, the inhibition by FR139317 of the catecholamine output responses was abolished by pretreatment with phosphoramidon (100 mM) or BQ-788 (3 μM). These results indicate that activation of endothelin ET(B) receptors by endogenous endothelins inhibits the catecholamine output responses under the condition in which endothelin ET(A) receptors are blocked. Exogenous endothelin-1 (1-100 nM) did not affect the catecholamine output responses, but it inhibited the responses under treatment with phosphoramidon and FR139317. Activation of endothelin ET(A) receptors may interfere with the endothelin ET(B) receptor-mediated inhibitory action on the neuronally evoked secretion of adrenal catecholamines. Copyright (C) 2000 Elsevier Science B.V.
AB - We investigated the role of endogenous endothelins in catecholamine secretion in response to transmural electrical stimulation in the retrogradely perfused rat adrenal gland. (R)2-[(R)-2-[(S)-2-[[1-(hexahydro-1H-azepinyl)]carbonyl]amino-4-methyl-pentanoyl]amino-3-[3-(1-methyl-1H-indoyl)]propionyl]amino-3-(2-pyridyl) propionic acid (FR139317; 0.03-3 μM), an endothelin ET(A) receptor antagonist, inhibited the electrical stimulation-induced epinephrine and norepinephrine output. Neither N-cis-2,6-dimethylpiperidinocarbonyl-L-γ-methylleucyl-D-1-methoxycarbonyltryptophanyl-D-norleucine (BQ-788; 0.03-3 μM), an endothelin ET(B) receptor antagonist, nor phosphoramidon (1-100 mM), an endothelin-converting enzyme inhibitor, affected the catecholamine output responses. However, the inhibition by FR139317 of the catecholamine output responses was abolished by pretreatment with phosphoramidon (100 mM) or BQ-788 (3 μM). These results indicate that activation of endothelin ET(B) receptors by endogenous endothelins inhibits the catecholamine output responses under the condition in which endothelin ET(A) receptors are blocked. Exogenous endothelin-1 (1-100 nM) did not affect the catecholamine output responses, but it inhibited the responses under treatment with phosphoramidon and FR139317. Activation of endothelin ET(A) receptors may interfere with the endothelin ET(B) receptor-mediated inhibitory action on the neuronally evoked secretion of adrenal catecholamines. Copyright (C) 2000 Elsevier Science B.V.
KW - BQ-788
KW - Endothelin-1
KW - FR139317
KW - Phosphoramidon
KW - Transmural electrical stimulation
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UR - http://www.scopus.com/inward/citedby.url?scp=0034613208&partnerID=8YFLogxK
U2 - 10.1016/S0014-2999(00)00651-8
DO - 10.1016/S0014-2999(00)00651-8
M3 - Article
C2 - 11011035
AN - SCOPUS:0034613208
VL - 406
SP - 69
EP - 74
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 1
ER -