Role of CU/ZN-SOD in the synthesis of endothelium-derived hyperpolarizing factor during reactive hyperemia in mouse mesenteric microvessels in vivo

Toyotaka Yada, Hiroaki Shimokawa, Keiko Morikawa, Aya Takaki, Masami Goto, Yasuo Ogasawara, Fumihiko Kajiya

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Background: We have recently identified that endothelium-derived hydrogen peroxide (H2O2) is an endothelium- derived hyperpolarlizing factor (EDHF) both in vitro and in vivo and that endothelial Cu/Zn-superoxide dismutase (SOD) plays an important role as an EDHF synthase in vitro. In this study, we examined the possible role of Cu/Zn-SOD in the synthesis of EDHF/ H2O2during reactive hyperemia in mouse mesenteric microcirculation in vivo. Methods: Mesenteric arterioles in wild-type and Cu/Zn-SOD-/-mice (n=6 each, 20-60 μm in diameter) were continuously observed by a newly developed pencil-typed intravital microscope during acetylcholine (ACh)-induced vasodilatation and reactive hyperemia (RH, reperfusion after 20 sec of mesenteric artery occlusion) under cyclooxygenase blockade (indomethacin, 5x10-5M, topically administration, TA) under the following 3 conditions; control, NO synthase inhibitor (L-NMMA, 10-4M, TA) and LNMMA+ catalase (PEG-catalase 10,000 U/kg, a specific decomposer of H2O2, intra-arterial administration). Results: In mesenteric arterioles of wild-type mice (n=6), endotheliumdependent relaxations to acetylcholine and vasodilatation during RH were totally resistant to L-NMMA, but were markedly inhibited by L-NMMA+catalase. In mesenteric arterioles of Cu/Zn-SOD-/-mice (n=6), L-NMMA markedly inhibited the relaxations to ACh and vasodilatation during RH. The responses were not inhibited by the addition of catalase. By contrast, endothelium-independent relaxations to sodium nitroprusside (10-4 M, TA), a direct NO donor, were comparable between the two strains in the presence of LNMMA+ catalase. Conclusions: Endothelial Cu/Zn-SOD plays an important role as an EDHF synthase during reactive hyperemia in mouse mesenteric microcirculation in vivo.

Original languageEnglish
Title of host publicationIFMBE Proceedings
EditorsSun I. Kim, Tae Suk Suh
PublisherSpringer Verlag
Pages3496-3499
Number of pages4
Edition1
ISBN (Print)9783540368397
DOIs
Publication statusPublished - 2007 Jan 1
Event10th World Congress on Medical Physics and Biomedical Engineering, WC 2006 - Seoul, Korea, Republic of
Duration: 2006 Aug 272006 Sep 1

Publication series

NameIFMBE Proceedings
Number1
Volume14
ISSN (Print)1680-0737
ISSN (Electronic)1433-9277

Other

Other10th World Congress on Medical Physics and Biomedical Engineering, WC 2006
CountryKorea, Republic of
CitySeoul
Period06/8/2706/9/1

Keywords

  • Cu/Zn-SOD
  • Endothelium-derived hyperpolarlizing factor
  • Hydrogen peroxide
  • Mouse mesenteric microcirculation
  • Reactive hyperemia

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering

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    Yada, T., Shimokawa, H., Morikawa, K., Takaki, A., Goto, M., Ogasawara, Y., & Kajiya, F. (2007). Role of CU/ZN-SOD in the synthesis of endothelium-derived hyperpolarizing factor during reactive hyperemia in mouse mesenteric microvessels in vivo. In S. I. Kim, & T. S. Suh (Eds.), IFMBE Proceedings (1 ed., pp. 3496-3499). (IFMBE Proceedings; Vol. 14, No. 1). Springer Verlag. https://doi.org/10.1007/978-3-540-36841-0_884