Role of chondroitin sulfate-hyaluronan interactions in the viscoelastic properties of extracellular matrices and fluids

Masahiro Nishimura, Weiqun Yan, Yoshiki Mukudai, Shigeo Nakamura, Kazuko Nakamasu, Masatoshi Kawata, Takeshi Kawamoto, Mitsuhide Noshiro, Taizo Hamada, Yukio Kato

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)

Abstract

The purpose of this study was to investigate the role of chondroitin sulfate-hyaluronan interactions in the viscoelastic properties of tissues and fluids, using capillary and cone-on-plate viscometers. Chondroitin sulfate markedly increased the viscosity of hyaluronan solutions at a wide range of hyaluronan mass (50-1900 kDa) under physiological conditions of pH, temperature, ionic strength and glycosaminoglycan concentration (0.5-40 mg/ml), although the viscosity of the chondroitin sulfate solutions themselves was very low. In the assay using a cone-on-plate viscometer, chondroitin sulfate increased the viscosity of hyaluronan solutions at various shear rates. At low shear rates, the viscosity of a chondroitin sulfate (5 mg/ml)-hyaluronan (0.5 mg/ml) mixture was about 40% of that of an aggrecan (5 mg/ml)-hyaluronan (0.5 mg/ml) mixture, and at 2.8-fold higher concentrations, chondroitin sulfate elicited the same effect on the viscosity of hyaluronan solutions (5 mg/ml) as an aggrecan monomer. In the presence of oscillatory motion, the addition of aggrecan increased the elasticity (storage) modulus G' and the viscosity (loss) modulus G'' of hyaluronan solutions and markedly decreased the loss tangent G''/ G' at frequencies corresponding to normal joint movements. In contrast, chondroitin sulfate had only a marginal effect on the loss tangent G''/ G', although it increased G' and G''. These findings demonstrated that chondroitin sulfate, as well as aggrecan, increases the viscosity of hyaluronan solutions, although chondroitin sulfate has less effect on the elasticity of hyaluronan solutions than that of aggrecan, and suggest that chondroitin sulfate may play an important physiological role in determining the viscoelastic properties of extracellular matrices and fluids.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalBiochimica et Biophysica Acta - General Subjects
Volume1380
Issue number1
DOIs
Publication statusPublished - 1998 Mar 12

Keywords

  • Chondroitin sulfate
  • Glycosaminoglycan
  • Hyaluronan
  • Viscosity

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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