Role of CC chemokines and their receptors in multiple aspects of mast cell biology: Comparative protein profiling of FcεRI- and/or CCR1-engaged mast cells using protein chip technology

Masako Toda, Takao Nakamura, Masaharu Ohbayashi, Yoshifumi Ikeda, Maria Dawson, Ricardo Micheler Richardson, Andrew Alban, Benjamin Leed, Dai Miyazaki, Santa Jeremy Ono

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Apart from the FcεRI-mediated mechanism, mast cells are activated by chemokines. Evidence has accumulated indicating that there is cross-talk between the FcεRI-mediated signalling pathway and CC chemokine receptor (CCR)-mediated signalling pathways in mast cells. We have found that costimulation with IgE/antigen and CC chemokine ligand 3 (CCL3) enhances degranulation but inhibits chemotaxis of rat basophilic leukaemia (RBL)-2H3 cells expressing human CCR1 (RBL-CCR1 cells). We hypothesize that this signalling cross-talk in mast cells may play important roles in the orchestration and focusing of the allergic response. In this study, we have sought information about global protein networks either enhanced or inhibited following cross-talk between the FcεRI-mediated and CCR-mediated signalling pathways in mast cells. We believe this information may be useful for providing an understanding of mast cell function and in the establishment of new anti-inflammatory drugs for allergic diseases. Proteomics is a promising tool for studying protein profiles within biological samples and facilitates an understanding of the complex responses of an organism to a stimulus. Here, we show comparative data of protein profiles derived from FcεRI-engaged and/or CCR1-engaged RBL-CCR1 cells using protein chip array technology, a proteomic technology. We also discuss our view of the role of CC chemokines and CCRs in regulating multiple aspects of mast cell biology.

Original languageEnglish
Title of host publicationMast Cells and Basophils
Subtitle of host publicationDevelopment, Activation and Roles in Allergic/Autoimmune Disease
Pages131-140
Number of pages10
Publication statusPublished - 2005 Dec 1
Externally publishedYes

Publication series

NameNovartis Foundation Symposium
Volume271
ISSN (Print)1528-2511

ASJC Scopus subject areas

  • Medicine(all)

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