Role of caveolin-1 and cytoskeletal proteins, actin and vimentin, in adipogenesis of bovine intramuscular preadipocyte cells

Takato Takenouchi, Norikazu Miyashita, Kyohei Ozutsumi, Michael T. Rose, Hisashi Aso

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

We investigated the involvement of caveolin-1 and the cytoskeletal proteins, actin and vimentin, in the adipogenesis of bovine intramuscular preadipocyte (BIP) cells. Immunoblot analysis demonstrated that levels of caveolin-1 and actin gradually increased during adipose conversion in BIP cells, whereas a slight decrease was observed for vimentin. We found that part of the vimentin was clearly distributed to caveolin-1-enriched membrane fractions in BIP cells, but actin was not. During adipogenesis of BIP cells, treatment with the tubulin depolymerizer, nocodazole, significantly increased intracellular triglyceride accumulation compared to non-treated cells. Immunocytochemical analysis showed that actin microfilaments were significantly disrupted in nocodazole-treated cells. Also, a decrease in the localization of vimentin in caveolin-1-enriched fractions and a failure of vimentin to co-immunoisolate with caveolin-1 were observed in nocodazole-treated cells. These results suggest that a rearrangement of cytoskeletal proteins has a role in the intracellular accumulation of lipid droplets during adipogenesis of BIP cells.

Original languageEnglish
Pages (from-to)615-623
Number of pages9
JournalCell Biology International
Volume28
Issue number8-9
DOIs
Publication statusPublished - 2004 Aug
Externally publishedYes

Keywords

  • Actin
  • Adipogenesis
  • Caveolin-1
  • Nocodazole
  • Vimentin

ASJC Scopus subject areas

  • Cell Biology

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