Role of Ca2+ transients at the node of the mouse embryo in breaking of left-right symmetry

Katsutoshi Mizuno, Kei Shiozawa, Takanobu A. Katoh, Katsura Minegishi, Takahiro Ide, Yayoi Ikawa, Hiromi Nishimura, Katsuyoshi Takaoka, Takeshi Itabashi, Atsuko H. Iwane, Junichi Nakai, Hidetaka Shiratori, Hiroshi Hamada

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Immotile cilia sense extracellular signals such as fluid flow, but whether Ca2+ plays a role in flow sensing has been unclear. Here, we examined the role of ciliary Ca2+ in the flow sensing that initiates the breaking of left-right (L-R) symmetry in the mouse embryo. Intraciliary and cytoplasmic Ca2+ transients were detected in the crown cells at the node. These Ca2+ transients showed L-R asymmetry, which was lost in the absence of fluid flow or the PKD2 channel. Further characterization allowed classification of the Ca2+ transients into two types: cilium-derived, L-R-asymmetric transients (type 1) and cilium-independent transients without an L-R bias (type 2). Type 1 intraciliary transients occurred preferentially at the left posterior region of the node, where L-R symmetry breaking takes place. Suppression of intraciliary Ca2+ transients delayed L-R symmetry breaking. Our results implicate cilium-derived Ca2+ transients in crown cells in initiation of L-R symmetry breaking in the mouse embryo.

Original languageEnglish
Article numberaba1195
JournalScience Advances
Volume6
Issue number30
DOIs
Publication statusPublished - 2020 Jul

ASJC Scopus subject areas

  • General

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