Role of Cav2.3 (αIE) Ca2+ channel in ischemic neuronal injury

Hideyuki Toriyama, Ling Wang, Hironao Saegusa, Shuqin Zong, Makoto Osanai, Takayuki Murakoshi, Tetsuo Noda, Kikuo Ohno, Tsutomu Tanabe

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We investigated the role of the Cav2.3 (αIE) channel in ischemic neuronal injury using Cav2.3 mutant mice. In focal ischemia model with a complete occlusion of the middle cerebral artery in vivo, infarct at 24 h was significantly larger in Cav2.3 mutant mice compared with that in wild-type controls. In vitro Ca2+ imaging studies using hippocampal slices revealed that oxygen-glucose deprivation induced a [Ca2+]i increase in the hippocampal CAI region more vigorously in Cav2.3 mutant mice than in wild-type controls, and that tetrodotoxin or bicuculline application abolished the difference between the genotypes. These results suggest that the Cav2.3 channel plays a protective role in ischemic neuronal injury by a mechanism in which GABAergic neuronal actions are involved.

Original languageEnglish
Pages (from-to)261-265
Number of pages5
JournalNeuroReport
Volume13
Issue number2
DOIs
Publication statusPublished - 2002 Feb 11

Keywords

  • Ca2.3 Ca channel
  • Hippocampal slice
  • Intracellular Ca transient
  • Ischemic neuronal injury
  • Knockout mouse
  • MCA occlusion

ASJC Scopus subject areas

  • Neuroscience(all)

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    Toriyama, H., Wang, L., Saegusa, H., Zong, S., Osanai, M., Murakoshi, T., Noda, T., Ohno, K., & Tanabe, T. (2002). Role of Cav2.3 (αIE) Ca2+ channel in ischemic neuronal injury. NeuroReport, 13(2), 261-265. https://doi.org/10.1097/00001756-200202110-00018