Role of cadherins in maintaining the compartment boundary between the cortex and striatum during development

T. Inoue, T. Tanaka, M. Takeichi, O. Chisaka, S. Nakamura, N. Osumi

Research output: Contribution to journalArticlepeer-review

138 Citations (Scopus)

Abstract

In ventricular cells of the mouse telencephalon, differential expression of cadherin cell adhesion molecules defines neighbouring regions; R-cadherin delineates the future cerebral cortex, while cadherin-6 delineates the lateral ganglionic eminence. By using cell labelling analyses in the whole embryo culture system, we demonstrated that the interface between R-cadherin and cadherin-6 expression is a boundary for cell lineage restriction at embryonic day 10.5. Interestingly, when a group of cells with exogenous cadherin-6 were generated to straddle the cortico-straital boundary by electroporation at embryonic day 11.0, ectopic cadherin-6-expressing cortical cells were sorted into the striatal compartment, and the reverse was the trend for ectopic R-cadherin-expressing striatal cells. Although cadherin-6 gene knockout mice engineered in this study showed no obvious phenotype in telencephalic compartmentalisation, the preferential sorting of ectopic cadherin-6-expressing cells was abolished in this mutant background. Thus, the differential expression pattern of cadherins in the embryonic telencephalon is responsible for maintaining the cortico-striatal compartment boundary.

Original languageEnglish
Pages (from-to)561-569
Number of pages9
JournalDevelopment
Volume128
Issue number4
Publication statusPublished - 2001

Keywords

  • Cadherins
  • Cell lineage restriction
  • Compartment
  • Cortico-striatal boundary
  • Electroporation
  • Mammalian whole embryo culture
  • Mouse
  • Neuromeres
  • Telencephalon

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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