Role of c-MYC On Erythroid Differentiation

Yasuhumi Ohmori, Jun ichi Tanabe, Masanori Terashima, Wataru Shoji, Shinji Takada, Masuo Obinata

Research output: Contribution to journalArticlepeer-review

Abstract

In the early event of the induction of mouse erythroleukemia (MEL) cell differentiation, c-myc mRNA levels show a biphasic change. The elevated expression of a transfected c-myc gene inhibits the commitment and differentiation of MEL cell transformants. In the present work, we have introduced human c-myc mutants into MEL cells under the inducible promoter to define the functional domains of c-Myc involved in erythroid differentiation. The c-Myc domains necessary for commitment and differentiation are not colocalized; almost entire regions are required for inhibition of commitment, whereas domains II and IV that are essential for co-transforming activity with ras are required for inhibition of differentiation. Interestingly, mutants that delete domains for c-Myc dimerization motifs enhanced differentiation. Thus, c-Myc interferes with MEL cell differentiation by interacting with c-Myc partners and the induced protein(s) through dimerization domains. These results suggest that c-Myc may regulate commitment and differentiation by interacting with proteins through different domains.

Original languageEnglish
Pages (from-to)203-210
Number of pages8
JournalTohoku Journal of Experimental Medicine
Volume168
Issue number2
DOIs
Publication statusPublished - 1992

Keywords

  • c-Myc
  • commitment
  • differentiation
  • dimerization domain
  • erythoroleukemia cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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