Abstract
In the early event of the induction of mouse erythroleukemia (MEL) cell differentiation, c-myc mRNA levels show a biphasic change. The elevated expression of a transfected c-myc gene inhibits the commitment and differentiation of MEL cell transformants. In the present work, we have introduced human c-myc mutants into MEL cells under the inducible promoter to define the functional domains of c-Myc involved in erythroid differentiation. The c-Myc domains necessary for commitment and differentiation are not colocalized; almost entire regions are required for inhibition of commitment, whereas domains II and IV that are essential for co-transforming activity with ras are required for inhibition of differentiation. Interestingly, mutants that delete domains for c-Myc dimerization motifs enhanced differentiation. Thus, c-Myc interferes with MEL cell differentiation by interacting with c-Myc partners and the induced protein(s) through dimerization domains. These results suggest that c-Myc may regulate commitment and differentiation by interacting with proteins through different domains.
Original language | English |
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Pages (from-to) | 203-210 |
Number of pages | 8 |
Journal | Tohoku Journal of Experimental Medicine |
Volume | 168 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1992 |
Externally published | Yes |
Keywords
- c-Myc
- commitment
- differentiation
- dimerization domain
- erythoroleukemia cells
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)