Role of bcl-2 in the development of lymphoid cells from the hematopoietic stem cell

Yumi Matsuzaki, Kei Ichi Nakayama, Keiko Nakayama, Takashi Tomita, Miu Isoda, Dennis Y. Loh, Hiromitsu Nakauchi

Research output: Contribution to journalArticlepeer-review

75 Citations (Scopus)

Abstract

To investigate the role of bcl-2 in lymphohematopoiesis, a long-term bone marrow reconstitution system was established. Transplantation of 1,000 c-Kit+ Sca-1+ and lineage markers negative cells from bcl-2-/- mouse bone marrow resulted in long-term reconstitution of nonlymphoid cells. However, T cells were totally absent and B-lymphocyte development was severely impaired at a very early stage of differentiation in the chimeric mouse. On the other hand, transplantation of day 14 fetal liver cells from bcl-2-/- mice resulted in generation of both T and B cells in the recipient, albeit transiently. These data suggest that bcl-2 plays a critical role in the development of lymphoid progenitor cells from the hematopoietic stem cell (HSC), but is not essential for the development of nonlymphoid cells and the self-renewal of HSC. In addition, lymphopoiesis from fetal liver HSC appears to be less dependent on bcl-2 than adult bone marrow HSC.

Original languageEnglish
Pages (from-to)853-862
Number of pages10
JournalBlood
Volume89
Issue number3
DOIs
Publication statusPublished - 1997 Feb 1

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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