Role of Aspergillus oryzae rolA in PBSA degradation

Toru Takahashi, Hiroshi Maeda, Sachiyo Yoneda, Shinsaku Ohtaki, Yohei Yamagata, Fumihiko Hasegawa, Masayuki Machida, Ryoji Ishioka, Katsuya Gomi, Keietsu Abe, Tasuku Nakajima

Research output: Contribution to conferencePaper

Abstract

When an industrial fungus Aspergillus oryzae was cultivated in liquid medium containing the biodegradable polyester polybutylene succinate-coadipate (PBSA), the rolA gene encoding hydrophobin RolA was highly transcribed. High levels of RolA and its localization on the cell surface in the presence of PBSA were confirmed by immunostaining. Under these conditions, A. oryzae simultaneously produced cutinase CutL1, which hydrolyzes PBSA. Preincubation of PBSA with RolA stimulated PBSA degradation by CutL1, suggesting that RolA bound to a PBSA surface was required for the stimulation. Immunostaining revealed that PBSA films coated with RolA specifically adsorbed CutL1. QCM analyses further demonstrated that RolA attached to a hydrophobic sensor chip specifically adsorbed CutL1. These results suggest that RolA adsorbed to the hydrophobic surface of PBSA recruits CutL1, resulting in condensation of CutLl on the PBSA surface and consequent stimulation of PBSA hydrolysis.

Original languageEnglish
Pages1948
Number of pages1
Publication statusPublished - 2005
Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
Duration: 2005 May 252005 May 27

Other

Other54th SPSJ Annual Meeting 2005
CountryJapan
CityYokohama
Period05/5/2505/5/27

Keywords

  • Aspergillus oryzae
  • Cutinase
  • Degradation
  • Hydrophobin
  • Interaction
  • PBSA
  • QCM

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Takahashi, T., Maeda, H., Yoneda, S., Ohtaki, S., Yamagata, Y., Hasegawa, F., Machida, M., Ishioka, R., Gomi, K., Abe, K., & Nakajima, T. (2005). Role of Aspergillus oryzae rolA in PBSA degradation. 1948. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.