Role of αv integrin in osteoprotegerin-induced endothelial cell migration and proliferation

Michiyo Kobayashi-Sakamoto, Emiko Isogai, Kimiharu Hirose, Itsuo Chiba

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Osteoprotegerin (OPG) is a decoy receptor for the receptor activator of nuclear factor κB ligand (RANKL). However, the role of OPG in the endothelium remains unknown. In this study, we demonstrate that OPG stimulates the proliferation and migration of human microvascular endothelial cells (HMVECs). In addition, we show that treatment with integrin αvβ3 or integrin αvβ5 blocking antibody inhibits endothelial cell migration. In contrast, treatment with anti-αvβ3 antibody or anti-αvβ5 antibody alone did not inhibit OPG-induced proliferation. However, OPG-induced proliferation was inhibited when these antibodies were applied simultaneously. Furthermore, OPG evoked activation of extracellular signal-regulated kinase (ERK) 1/2, which has been linked to integrin αv activity. Taken together, these results suggest that integrins αvβ3 and/or αvβ5 contribute to endothelial cell proliferation and migration induced by OPG.

Original languageEnglish
Pages (from-to)139-144
Number of pages6
JournalMicrovascular Research
Volume76
Issue number3
DOIs
Publication statusPublished - 2008 Nov 1

Keywords

  • Endothelial cells
  • Extracellular signal-regulated kinase
  • Integrin αβ
  • Migration
  • Osteoprotegerin
  • Proliferation
  • integrin αβ

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology

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