RNAi screening of human glycogene orthologs in the nematode Caenorhabditis elegans and the construction of the C. elegans glycogene database

Sayaka Akiyoshi, Kazuko H. Nomura, Katsufumi Dejima, Daisuke Murata, Ayako Matsuda, Nanako Kanaki, Tetsuro Takaki, Hiroyuki Mihara, Takayuki Nagaishi, Shuhei Furukawa, Keiko Gengyo Ando, Sawako Yoshina, Shohei Mitani, Akira Togayachi, Yoshinori Suzuki, Toshihide Shikanai, Hisashi Narimatsu, Kazuya Nomura

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

In this study, we selected 181 nematode glycogenes that are orthologous to human glycogenes and examined their RNAi phenotypes. The results are deposited in the Caenorhabditis elegans Glycogene Database (CGGDB) at AIST, Tsukuba, Japan. The most prominent RNAi phenotypes observed are disruptions of cell cycle progression in germline mitosis/meiosis and in early embryonic cell mitosis. Along with the previously reported roles of chondroitin proteoglycans, glycosphingolipids and GPI-anchored proteins in cell cycle progression, we show for the first time that the inhibition of the functions of N-glycan synthesis genes (cytoplasmic alg genes) resulted in abnormal germline formation, ER stress and small body size phenotypes. The results provide additional information on the roles of glycoconjugates in the cell cycle progression mechanisms of germline and embryonic cells.

Original languageEnglish
Pages (from-to)8-20
Number of pages13
JournalGlycobiology
Volume25
Issue number1
DOIs
Publication statusPublished - 2015 Jan 1

Keywords

  • RNAi
  • alg genes
  • germline cell division
  • glycogene
  • oligosaccharyltransferase

ASJC Scopus subject areas

  • Biochemistry

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