RNA interference-mediated silencing of synaptotagmin IX, but not synaptotagmin I, inhibits dense-core vesicle exocytosis in PC12 cells

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36 Citations (Scopus)

Abstract

Although PC12 cells express three synaptotagmin isoforms (Syts I, IV and IX), all of which have been proposed to regulate dense-core vesicle exocytosis, it remains unknown which of the Syt isoforms acts as the major Ca2+ sensor for dense-core vesicle exocytosis. In the present study, it has been shown by immunoaffinity purification and immunocytochemistry that Syts I and IX, but not Syt IV, are present on the same secretory vesicles in PC12 cells. Silencing of Syt IX with specific small interfering RNA significantly reduced high KC1-dependent neuropeptide Y secretion from PC12 cells, whereas silencing of Syt I with specific small interfering RNA had no significant effect. The results indicate that Syts I and IX are not functionally equivalent and that Syt IX, and not Syt I, is indispensable for the regulation of Ca 2+-dependent dense-core vesicle exocytosis in PC12 cells.

Original languageEnglish
Pages (from-to)875-879
Number of pages5
JournalBiochemical Journal
Volume380
Issue number3
DOIs
Publication statusPublished - 2004 Jun 15

Keywords

  • Ca sensor
  • Double-stranded RNA-mediated interference (RNAi)
  • Endocrine cell
  • Exocytosis
  • PC12 cell
  • Synaptotagmin

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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