RIM1 confers sustained activity and neurotransmitter vesicle anchoring to presynaptic Ca2+ channels

Shigeki Kiyonaka, Minoru Wakamori, Takafumi Miki, Yoshitsugu Uriu, Mio Nonaka, Haruhiko Bito, Aaron M. Beedle, Emiko Mori, Yuji Hara, Michel De Waard, Motoi Kanagawa, Makoto Itakura, Masami Takahashi, Kevin P. Campbell, Yasuo Mori

Research output: Contribution to journalArticlepeer-review

173 Citations (Scopus)

Abstract

The molecular organization of presynaptic active zones is important for the neurotransmitter release that is triggered by depolarization-induced Ca 2+ influx. Here, we demonstrate a previously unknown interaction between two components of the presynaptic active zone, RIM1 and voltage-dependent Ca2+ channels (VDCCs), that controls neurotransmitter release in mammalian neurons. RIM1 associated with VDCC β-subunits via its C terminus to markedly suppress voltage-dependent inactivation among different neuronal VDCCs. Consistently, in pheochromocytoma neuroendocrine PC12 cells, acetylcholine release was significantly potentiated by the full-length and C-terminal RIM1 constructs, but membrane docking of vesicles was enhanced only by the full-length RIM1. The β construct beta-AID dominant negative, which disrupts the RIM1-β association, accelerated the inactivation of native VDCC currents, suppressed vesicle docking and acetylcholine release in PC12 cells, and inhibited glutamate release in cultured cerebellar neurons. Thus, RIM1 association with β in the presynaptic active zone supports release via two distinct mechanisms: sustaining Ca2+ influx through inhibition of channel inactivation, and anchoring neurotransmitter-containing vesicles in the vicinity of VDCCs.

Original languageEnglish
Pages (from-to)691-701
Number of pages11
JournalNature Neuroscience
Volume10
Issue number6
DOIs
Publication statusPublished - 2007 Jun
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)

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