rig encodes ribosomal protein S15 The primary structure of mammalian ribosomal protein S15

Motoo Kitagawa, Shin Takasawa, Norihisa Kikuchi, Takako Itoh, Hiroshi Teraoka, Hiroshi Yamamoto, Hiroshi Okamoto

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)

Abstract

rig, a gene originally isolated from a rat insulinoma cDNA library, codes for a basic 145 amino acid protein [(1986) Diabetes 35, 1178-1180]. Here we show that the immunoreactivity to a monocional antibody against the deduced rig protein and the translation product of rig mRNA comigrated with ribosomal protein S15. The amino acid sequence of ribosomal protein S15 purified from rat liver coincided with that deduced from the nucleotide sequence of rig mRNA, but there were indications that the initiator methionine was removed and the succeeding alanyl residue was monoacetylated. From these results, we conclude that the product of rig is ribosomal protein S15.

Original languageEnglish
Pages (from-to)210-214
Number of pages5
JournalFEBS Letters
Volume283
Issue number2
DOIs
Publication statusPublished - 1991 Jun 3

Keywords

  • Amino acid sequence
  • N-terminal modification
  • Rat liver
  • Ribosomal protein S15
  • rig
  • rig protein

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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