Rice phenolics efflux transporter 2 (PEZ2) plays an important role in solubilizing apoplasmic iron

Khurram Bashir, Yasuhiro Ishimaru, Hugo Shimo, Yusuke Kakei, Takeshi Senoura, Ryuichi Takahashi, Yutaka Sato, Yuki Sato, Nobuyuki Uozumi, Hiromi Nakanishi, Naoko K. Nishizawa

Research output: Contribution to journalArticlepeer-review

49 Citations (Scopus)

Abstract

Iron (Fe) is an essential micronutrient for plants; however, despite its abundance in soils it is not readily available in neutral to alkaline soils. Plants secrete phenolics, such as protocatechuic acid (PCA) and caffeic acid (CA), to absorb and utilize precipitated apoplasmic Fe from root surfaces. However, the synthesis and secretion of phenolics have not been well characterized in plants. We have identified and characterized a rice (Oryza sativa L.) mutant with reduced amounts of PCA and CA in xylem sap and root exudates; hence we named it phenolics efflux zero 2 (pez2). PEZ2 localized to the plasma membrane in onion (Allium cepa L.) epidermal cells and transported PCA when expressed in Xenopus laevis oocytes. PEZ2 expression was observed in whole root near root tips. Similarly, strong expression was observed in leaves. In line with reduced amounts of PCA and CA in xylem sap, the xylem Fe concentration was also low in pez2 plants. These results suggest that PEZ2 is involved in solubilization of apoplasmic Fe in rice.

Original languageEnglish
Pages (from-to)803-812
Number of pages10
JournalSoil Science and Plant Nutrition
Volume57
Issue number6
DOIs
Publication statusPublished - 2011

Keywords

  • Iron
  • Iron transport
  • Phenolics transporter
  • Protocatechuic acid
  • Xylem sap

ASJC Scopus subject areas

  • Soil Science
  • Plant Science

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