Rice metal-nicotianamine transporter, OsYSL2, is required for the long-distance transport of iron and manganese

Yasuhiro Ishimaru, Hiroshi Masuda, Khurram Bashir, Haruhiko Inoue, Takashi Tsukamoto, Michiko Takahashi, Hiromi Nakanishi, Naohiro Aoki, Tatsuro Hirose, Ryu Ohsugi, Naoko K. Nishizawa

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209 Citations (Scopus)

Abstract

Rice (Oryza sativa) is indispensable in the diet of most of the world's population. Thus, it is an important target in which to alter iron (Fe) uptake and homeostasis, so as to increase Fe accumulation in the grain. We previously isolated OsYSL2, a functional iron [Fe(II)]- and manganese [Mn(II)]- nicotianamine complex transporter that is expressed in phloem cells and developing seeds. We produced RNAi (OsYSL2i) and overexpression lines (OXOsYSL2) of OsYSL2. At the vegetative stage in an OsYSL2i line, the Fe and Mn concentrations were decreased in the shoots, and the Fe concentration was increased in the roots. At the reproductive stage, positron-emitting tracer imaging system analysis revealed that Fe translocation to the shoots and seeds was suppressed in OsYSL2i. The Fe and Mn concentrations were decreased in the seeds of OsYSL2i, especially in the endosperm. Moreover, the Fe concentration in OXOsYSL2 was lower in the seeds and shoots, but higher in the roots, compared with the wild type. Furthermore, when OsYSL2 expression was driven by the sucrose transporter promoter, the Fe concentration in the polished rice was up to 4.4-fold higher compared with the wild type. These results indicate that the altered expression of OsYSL2 changes the localization of Fe, and that OsYSL2 is a critical Fe-nicotianamine transporter important for Fe translocation, especially in the shoots and endosperm.

Original languageEnglish
Pages (from-to)379-390
Number of pages12
JournalPlant Journal
Volume62
Issue number3
DOIs
Publication statusPublished - 2010 May 1
Externally publishedYes

Keywords

  • Endosperm
  • Iron
  • Manganese
  • Nicotianamine
  • Rice
  • Yellow stripe 1

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

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    Ishimaru, Y., Masuda, H., Bashir, K., Inoue, H., Tsukamoto, T., Takahashi, M., Nakanishi, H., Aoki, N., Hirose, T., Ohsugi, R., & Nishizawa, N. K. (2010). Rice metal-nicotianamine transporter, OsYSL2, is required for the long-distance transport of iron and manganese. Plant Journal, 62(3), 379-390. https://doi.org/10.1111/j.1365-313X.2010.04158.x