Rice DEP1, encoding a highly cysteine-rich G protein γ subunit, confers cadmium tolerance on yeast cells and plants

Shuta Kunihiro, Tatsuhiko Saito, Taiki Matsuda, Masataka Inoue, Masato Kuramata, Fumio Taguchi-Shiobara, Shohab Youssefian, Thomas Berberich, Tomonobu Kusano

    Research output: Contribution to journalArticle

    29 Citations (Scopus)

    Abstract

    A rice cDNA, OsDEP1, encoding a highly cysteine (Cys)-rich G protein γ subunit, was initially identified as it conferred cadmium (Cd) tolerance on yeast cells. Of the 426 aa constituting OsDEP1, 120 are Cys residues (28.2%), of which 88 are clustered in the C-terminal half region (aa 170-426). To evaluate the independent effects of these two regions, two truncated versions of the OsDEP1-expressing plasmids pOsDEP1(1-169) and pOsDEP1(170-426) were used to examine their effects on yeast Cd tolerance. Although OsDEP1(170-426) conferred a similar level of Cd tolerance as the intact OsDEP1, OsDEP1(1-169) provided no such tolerance, indicating that the tolerance effect is localized to the aa 170-426 C-terminal peptide region. The Cd responses of transgenic Arabidopsis plants constitutively expressing OsDEP1, OsDEP1(1-169) or OsDEP1(170-426), were similar to the observations in yeast cells, with OsDEP1 and OsDEP1(170-426) transgenic plants displaying Cd tolerance but OsDEP1(1-169) plants showing no such tolerance. In addition, a positive correlation between the transcript levels of OsDEP1 or OsDEP1(170-426) in the transgenics and the Cd content of these plants upon Cd application was observed. As several Arabidopsis loss-of-function heterotri-meric G protein β and γ subunit gene mutants did not show differences in their Cd sensitivity compared with wild-type plants, we propose that the Cys-rich region of OsDEP1 may function directly as a trap for Cd ions.

    Original languageEnglish
    Pages (from-to)4517-4527
    Number of pages11
    JournalJournal of Experimental Botany
    Volume64
    Issue number14
    DOIs
    Publication statusPublished - 2013 Nov 1

    Keywords

    • Cadmium tolerance
    • Cysteine-rich protein
    • G protein γsubunit
    • Heterotrimeric G protein signalling
    • Oryza sativa
    • OsDEP1

    ASJC Scopus subject areas

    • Physiology
    • Plant Science

    Fingerprint Dive into the research topics of 'Rice DEP1, encoding a highly cysteine-rich G protein γ subunit, confers cadmium tolerance on yeast cells and plants'. Together they form a unique fingerprint.

  • Cite this

    Kunihiro, S., Saito, T., Matsuda, T., Inoue, M., Kuramata, M., Taguchi-Shiobara, F., Youssefian, S., Berberich, T., & Kusano, T. (2013). Rice DEP1, encoding a highly cysteine-rich G protein γ subunit, confers cadmium tolerance on yeast cells and plants. Journal of Experimental Botany, 64(14), 4517-4527. https://doi.org/10.1093/jxb/ert267