Ribosome biogenesis factors bind a nuclear envelope SUN domain protein to cluster yeast telomeres

Chihiro Horigome, Takafumi Okada, Kyoko Shimazu, Susan M. Gasser, Keiko Mizuta

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

Two interacting ribosome biogenesis factors, Ebp2 and Rrs1, associate with Mps3, an essential inner nuclear membrane protein. Both are found in foci along the nuclear periphery, like Mps3, as well as in the nucleolus. Temperature-sensitive ebp2 and rrs1 mutations that compromise ribosome biogenesis displace the mutant proteins from the nuclear rim and lead to a distorted nuclear shape. Mps3 is known to contribute to the S-phase anchoring of telomeres through its interaction with the silent information regulator Sir4 and yKu. Intriguingly, we find that both Ebp2 and Rrs1 interact with the C-terminal domain of Sir4, and that conditional inactivation of either ebp2 or rrs1 interferes with both the clustering and silencing of yeast telomeres, while telomere tethering to the nuclear periphery remains intact. Importantly, expression of an Ebp2ĝ€"Mps3 fusion protein in the ebp2 mutant suppresses the defect in telomere clustering, but not its defects in growth or ribosome biogenesis. Our results suggest that the ribosome biogenesis factors Ebp2 and Rrs1 cooperate with Mps3 to mediate telomere clustering, but not telomere tethering, by binding Sir4.

Original languageEnglish
Pages (from-to)3799-3811
Number of pages13
JournalEMBO Journal
Volume30
Issue number18
DOIs
Publication statusPublished - 2011 Sep
Externally publishedYes

Keywords

  • nuclear envelope
  • ribosome biogenesis
  • SUN protein
  • telomere clusters
  • telomeric silencing

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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