Rhophilin, a small GTPase Rho-binding protein, is abundantly expressed in the mouse testis and localized in the principal piece of the sperm tail

Ken Ichi Nakamura; Akiko Fujita; Takahiko Murata; Go Watanabe; Chisato Mori; Jun Fujita; Naoki Watanabe; Toshimasa Ishizaki; Osamu Yoshida; Shuh Narumiya

doi:10.1016/S0014-5793(99)00087-3

Rhophilin, a small GTPase Rho-binding protein, is abundantly expressed in the mouse testis and localized in the principal piece of the sperm tail

Ken Ichi Nakamura, Akiko Fujita, Takahiko Murata, Go Watanabe, Chisato Mori, Jun Fujita, Naoki Watanabe, Toshimasa Ishizaki, Osamu Yoshida, Shuh Narumiya

Research output: Contribution to journal › Article › peer-review

60 Citations (Scopus)

Abstract

Tissue distribution and cellular localization of rhophilin, a 71 kDa Rho-binding protein, were examined in mice. Rhophilin mRNA was highly expressed in adult testis, but was absent in the testis of W/W(V) mice deficient in germ cells. An anti-rhophilin antibody detected a band of an expected size in sperm extracts, which was enriched in the tail fraction. Immunofluorescence analysis revealed two lines of striated staining running in parallel in the principal piece of the sperm tail. These results suggest that rhophilin is expressed in germ cells and localized in the fibrous sheath of the sperm tail. Copyright (C) 1999 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	9-13
Number of pages	5
Journal	FEBS Letters
Volume	445
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(99)00087-3
Publication status	Published - 1999 Feb 19
Externally published	Yes

Keywords

Fibrous sheath
Rho
Rhophilin
Sperm
Sperm tail
Testis

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(99)00087-3

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title = "Rhophilin, a small GTPase Rho-binding protein, is abundantly expressed in the mouse testis and localized in the principal piece of the sperm tail",

abstract = "Tissue distribution and cellular localization of rhophilin, a 71 kDa Rho-binding protein, were examined in mice. Rhophilin mRNA was highly expressed in adult testis, but was absent in the testis of W/W(V) mice deficient in germ cells. An anti-rhophilin antibody detected a band of an expected size in sperm extracts, which was enriched in the tail fraction. Immunofluorescence analysis revealed two lines of striated staining running in parallel in the principal piece of the sperm tail. These results suggest that rhophilin is expressed in germ cells and localized in the fibrous sheath of the sperm tail. Copyright (C) 1999 Federation of European Biochemical Societies.",

keywords = "Fibrous sheath, Rho, Rhophilin, Sperm, Sperm tail, Testis",

author = "Nakamura, {Ken Ichi} and Akiko Fujita and Takahiko Murata and Go Watanabe and Chisato Mori and Jun Fujita and Naoki Watanabe and Toshimasa Ishizaki and Osamu Yoshida and Shuh Narumiya",

note = "Funding Information: We would like to thank M. Maekawa, H. Bito, P. Madaule and T. Terachi for their valuable discussions and advices, K. Nonomura for the technical assistance and T. Arai and H. Nose for the secretarial assistance. This work was supported in part by a Grant-in-Aid for Specially Promoted Research (08102007) from the Ministry of Education, Science and Culture of Japan, and by the Human Frontier Science Program.",

year = "1999",

month = feb,

day = "19",

doi = "10.1016/S0014-5793(99)00087-3",

language = "English",

volume = "445",

pages = "9--13",

journal = "FEBS Letters",

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TY - JOUR

T1 - Rhophilin, a small GTPase Rho-binding protein, is abundantly expressed in the mouse testis and localized in the principal piece of the sperm tail

AU - Nakamura, Ken Ichi

AU - Fujita, Akiko

AU - Murata, Takahiko

AU - Watanabe, Go

AU - Mori, Chisato

AU - Fujita, Jun

AU - Watanabe, Naoki

AU - Ishizaki, Toshimasa

AU - Yoshida, Osamu

AU - Narumiya, Shuh

N1 - Funding Information: We would like to thank M. Maekawa, H. Bito, P. Madaule and T. Terachi for their valuable discussions and advices, K. Nonomura for the technical assistance and T. Arai and H. Nose for the secretarial assistance. This work was supported in part by a Grant-in-Aid for Specially Promoted Research (08102007) from the Ministry of Education, Science and Culture of Japan, and by the Human Frontier Science Program.

PY - 1999/2/19

Y1 - 1999/2/19

N2 - Tissue distribution and cellular localization of rhophilin, a 71 kDa Rho-binding protein, were examined in mice. Rhophilin mRNA was highly expressed in adult testis, but was absent in the testis of W/W(V) mice deficient in germ cells. An anti-rhophilin antibody detected a band of an expected size in sperm extracts, which was enriched in the tail fraction. Immunofluorescence analysis revealed two lines of striated staining running in parallel in the principal piece of the sperm tail. These results suggest that rhophilin is expressed in germ cells and localized in the fibrous sheath of the sperm tail. Copyright (C) 1999 Federation of European Biochemical Societies.

AB - Tissue distribution and cellular localization of rhophilin, a 71 kDa Rho-binding protein, were examined in mice. Rhophilin mRNA was highly expressed in adult testis, but was absent in the testis of W/W(V) mice deficient in germ cells. An anti-rhophilin antibody detected a band of an expected size in sperm extracts, which was enriched in the tail fraction. Immunofluorescence analysis revealed two lines of striated staining running in parallel in the principal piece of the sperm tail. These results suggest that rhophilin is expressed in germ cells and localized in the fibrous sheath of the sperm tail. Copyright (C) 1999 Federation of European Biochemical Societies.

KW - Fibrous sheath

KW - Rho

KW - Rhophilin

KW - Sperm

KW - Sperm tail

KW - Testis

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AN - SCOPUS:0032992519

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Rhophilin, a small GTPase Rho-binding protein, is abundantly expressed in the mouse testis and localized in the principal piece of the sperm tail

Abstract

Keywords

ASJC Scopus subject areas

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