Rhophilin, a small GTPase Rho-binding protein, is abundantly expressed in the mouse testis and localized in the principal piece of the sperm tail

Ken Ichi Nakamura, Akiko Fujita, Takahiko Murata, Go Watanabe, Chisato Mori, Jun Fujita, Naoki Watanabe, Toshimasa Ishizaki, Osamu Yoshida, Shuh Narumiya

    Research output: Contribution to journalArticlepeer-review

    56 Citations (Scopus)

    Abstract

    Tissue distribution and cellular localization of rhophilin, a 71 kDa Rho-binding protein, were examined in mice. Rhophilin mRNA was highly expressed in adult testis, but was absent in the testis of W/W(V) mice deficient in germ cells. An anti-rhophilin antibody detected a band of an expected size in sperm extracts, which was enriched in the tail fraction. Immunofluorescence analysis revealed two lines of striated staining running in parallel in the principal piece of the sperm tail. These results suggest that rhophilin is expressed in germ cells and localized in the fibrous sheath of the sperm tail. Copyright (C) 1999 Federation of European Biochemical Societies.

    Original languageEnglish
    Pages (from-to)9-13
    Number of pages5
    JournalFEBS Letters
    Volume445
    Issue number1
    DOIs
    Publication statusPublished - 1999 Feb 19

    Keywords

    • Fibrous sheath
    • Rho
    • Rhophilin
    • Sperm
    • Sperm tail
    • Testis

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology

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