Abstract
Tissue distribution and cellular localization of rhophilin, a 71 kDa Rho-binding protein, were examined in mice. Rhophilin mRNA was highly expressed in adult testis, but was absent in the testis of W/W(V) mice deficient in germ cells. An anti-rhophilin antibody detected a band of an expected size in sperm extracts, which was enriched in the tail fraction. Immunofluorescence analysis revealed two lines of striated staining running in parallel in the principal piece of the sperm tail. These results suggest that rhophilin is expressed in germ cells and localized in the fibrous sheath of the sperm tail. Copyright (C) 1999 Federation of European Biochemical Societies.
Original language | English |
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Pages (from-to) | 9-13 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 445 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1999 Feb 19 |
Externally published | Yes |
Keywords
- Fibrous sheath
- Rho
- Rhophilin
- Sperm
- Sperm tail
- Testis
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology