TY - JOUR
T1 - Rhizosphere modelling reveals spatiotemporal distribution of daidzein shaping soybean rhizosphere bacterial community
AU - Okutani, Fuki
AU - Hamamoto, Shoichiro
AU - Aoki, Yuichi
AU - Nakayasu, Masaru
AU - Nihei, Naoto
AU - Nishimura, Taku
AU - Yazaki, Kazufumi
AU - Sugiyama, Akifumi
N1 - Funding Information:
We thank Ms Yuko Kobayashi and Mr Takahito Tastuno for technical assistance. We thank Dr Hisabumi Takase of the Kyoto University of Advanced Science for assistance with soil sampling. We also thank DASH/FBAS, the Research Institute for Sustainable Humanosphere, Kyoto University, and Kyoto Prefectural Agriculture, Forestry and Fisheries Technology Research Center for the soybean seeds. This work was supported in part by JST CREST Grant JPMJCR17O2 (to Y. A. and A. S.), JSPS KAKENHI Grant 18H02313 (to S. H. and A. S.), and funds from the Research Institute for Sustainable Humanosphere and the Research Unit for Development of Global Sustainability, Kyoto University (to A. S.).
Funding Information:
We thank Ms Yuko Kobayashi and Mr Takahito Tastuno for technical assistance. We thank Dr Hisabumi Takase of the Kyoto University of Advanced Science for assistance with soil sampling. We also thank DASH/FBAS, the Research Institute for Sustainable Humanosphere, Kyoto University, and Kyoto Prefectural Agriculture, Forestry and Fisheries Technology Research Center for the soybean seeds. This work was supported in part by JST CREST Grant JPMJCR17O2 (to Y. A. and A. S.), JSPS KAKENHI Grant 18H02313 (to S. H. and A. S.), and funds from the Research Institute for Sustainable Humanosphere and the Research Unit for Development of Global Sustainability, Kyoto University (to A. S.).
Publisher Copyright:
© 2019 John Wiley & Sons Ltd.
PY - 2020/4/1
Y1 - 2020/4/1
N2 - Plant roots nurture a wide variety of microbes via exudation of metabolites, shaping the rhizosphere's microbial community. Despite the importance of plant specialized metabolites in the assemblage and function of microbial communities in the rhizosphere, little is known of how far the effects of these metabolites extend through the soil. We employed a fluid model to simulate the spatiotemporal distribution of daidzein, an isoflavone secreted from soybean roots, and validated using soybeans grown in a rhizobox. We then analysed how daidzein affects bacterial communities using soils artificially treated with daidzein. Simulation of daidzein distribution showed that it was only present within a few millimetres of root surfaces. After 14 days in a rhizobox, daidzein was only present within 2 mm of root surfaces. Soils with different concentrations of daidzein showed different community composition, with reduced α-diversity in daidzein-treated soils. Bacterial communities of daidzein-treated soils were closer to those of the soybean rhizosphere than those of bulk soils. This study highlighted the limited distribution of daidzein within a few millimetres of root surfaces and demonstrated a novel role of daidzein in assembling bacterial communities in the rhizosphere by acting as more of a repellant than an attractant.
AB - Plant roots nurture a wide variety of microbes via exudation of metabolites, shaping the rhizosphere's microbial community. Despite the importance of plant specialized metabolites in the assemblage and function of microbial communities in the rhizosphere, little is known of how far the effects of these metabolites extend through the soil. We employed a fluid model to simulate the spatiotemporal distribution of daidzein, an isoflavone secreted from soybean roots, and validated using soybeans grown in a rhizobox. We then analysed how daidzein affects bacterial communities using soils artificially treated with daidzein. Simulation of daidzein distribution showed that it was only present within a few millimetres of root surfaces. After 14 days in a rhizobox, daidzein was only present within 2 mm of root surfaces. Soils with different concentrations of daidzein showed different community composition, with reduced α-diversity in daidzein-treated soils. Bacterial communities of daidzein-treated soils were closer to those of the soybean rhizosphere than those of bulk soils. This study highlighted the limited distribution of daidzein within a few millimetres of root surfaces and demonstrated a novel role of daidzein in assembling bacterial communities in the rhizosphere by acting as more of a repellant than an attractant.
KW - advection–diffusion equation
KW - bacterial communities
KW - rhizosphere modelling
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U2 - 10.1111/pce.13708
DO - 10.1111/pce.13708
M3 - Article
C2 - 31875335
AN - SCOPUS:85077840022
VL - 43
SP - 1036
EP - 1046
JO - Plant, Cell and Environment
JF - Plant, Cell and Environment
SN - 0140-7791
IS - 4
ER -