Rhizobitoxine inhibition of hydrogenase synthesis in free-living Bradyrhizobium japonicum

K. Minamisawa, K. Fukai, T. Asami

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Rhizobitoxine produced by Bradyrhizobium species strongly prevented derepression of hydrogenase expression in free-living Bradyrhizobium japonicum, although the toxin had no effect on the activity of cells which had already synthesized hydrogenase protein. Dihydrorhizobitoxine, a structural analog of rhizobitoxine, proved to be a less potent inhibitor of hydrogenase derepression. Rhizobitoxine did not cause cell death at a concentraation sufficient to eliminate hydrogenase expression. The large subunit of hydrogenase was not detectable with antibody after derepression in the presence of rhizobitoxine. The general pattern of proteins synthesized from 14C-labeled amino acids during derepression was not significantly different in the presence or absence of rhizohiboxine. These results indicated that rhizobitoxine inhibited hydrogenase synthesis in free-living B. japonicum. Cystathionine and methionine strongly prevented the inhibition of hydrogenase derepression by rhizobitoxine, suggesting that the inhibition involves the level of sulfur-containing amino acids in the cell.

Original languageEnglish
Pages (from-to)4505-4509
Number of pages5
JournalJournal of bacteriology
Volume172
Issue number8
DOIs
Publication statusPublished - 1990

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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