Rhizobial infection does not require cortical expression of upstream common symbiosis genes responsible for the induction of Ca2+ spiking

Teruyuki Hayashi, Yoshikazu Shimoda, Shusei Sato, Satoshi Tabata, Haruko Imaizumi-Anraku, Makoto Hayashi

    Research output: Contribution to journalArticlepeer-review

    34 Citations (Scopus)

    Abstract

    For the establishment of an effective root nodule symbiosis, a coordinated regulation of the infection processes between the epidermis and cortex is required. However, it remains unclear whether the symbiotic genes identified so far are involved in epidermal and/or cortical infection, e.g. epidermal and cortical infection thread formation or cortical cell division. To analyze the symbiotic gene requirements of the infection process, we have developed an epidermis-specific expression system (pEpi expression system) and examined the symbiotic genes NFR1, NFR5, NUP85, NUP133, CASTOR, POLLUX, CCaMK, CYCLOPS, NSP1 and NSP2 for involvement in the infection process in the epidermis and cortex. Our study shows that expression of the upstream common symbiosis genes CASTOR, POLLUX, NUP85 and NUP133 in the epidermis is sufficient to induce formation of infection threads and cortical cell division, leading to the development of fully effective nodules. Our system also shows a requirement of CCaMK, CYCLOPS, NSP1 and NSP2 for the entire nodulation process, and the different contributions of NFR1 and NFR5 to cortical infection thread formation. Based on these analyses using the pEpi expression system, we propose a functional model of symbiotic genes for epidermal and cortical infection.

    Original languageEnglish
    Pages (from-to)146-159
    Number of pages14
    JournalPlant Journal
    Volume77
    Issue number1
    DOIs
    Publication statusPublished - 2014 Jan

    Keywords

    • Lotus japonicus
    • cortical cell division
    • epidermis-specific expression system (pEpi expression system)
    • infection threads
    • root cortex
    • root epidermis
    • root nodule symbiosis

    ASJC Scopus subject areas

    • Genetics
    • Plant Science
    • Cell Biology

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