Rhipicephalus appendiculatus: Characterization of a testis-associated protein

Shinji Yamada, Yuko Ito, Saiki Imamura, Satoru Konnai, Takuya Ito, Misao Onuma, Kazuhiko Ohashi

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

A novel gene coding for Rhipicephalus appendiculatus Male-specific Protein (RAMP) was identified in a cDNA library constructed from the testis/vas deferens of R. appendiculatus ticks. This gene encodes a secreted protein exclusively expressed in the testis/vas deferens. The putative RAMP amino acid sequence contains a signal peptide and has 29% amino acid identity with male-specific Is5 gene of Ixodes scapularis. Gene expression studies revealed that RAMP mRNA was up-regulated in male ticks during blood feeding. RAMP was detected not only in the testis/vas deferens of males but also in postcoitum female ticks based on Western blotting, indicating that this protein is transferred to the female tick during copulation. Virgin female ticks, microinjected with recombinant RAMP, had significantly prolonged attachment duration during feeding, but there was no effect on fed weight. These results suggest that RAMP is a male-specific molecule in the spermatophore, and is related to female attachment behavior in R. appendiculatus ticks.

Original languageEnglish
Pages (from-to)337-342
Number of pages6
JournalExperimental Parasitology
Volume120
Issue number4
DOIs
Publication statusPublished - 2008 Dec

Keywords

  • Rhipicephalus appendiculatus
  • Testis/vas deferens
  • Tick mating
  • cDNA library

ASJC Scopus subject areas

  • Parasitology
  • Immunology
  • Infectious Diseases

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