Rhamnose-binding lectins induce respiratory burst activity in macrophage cells from rainbow trout

Yasuharu Watanabe, Yin Hsuan Chang, Osamu Nakamura, Takako Naganuma, Tomohisa Ogawa, Koji Muramoto

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Rhamnose-binding lectins (RBLs) have been isolated and characterized from various kinds of fish. RBLs recognize the structures of lipopolysaccharides and lipoteichoic acid on microbes, and have an opsonic effect, suggesting that RBLs play an important role as pattern recognition proteins in innate immunity. However, the function of RBLs in the removal of pathogens from the body has not yet been elucidated. In this study, we investigated the effect of three RBLs-named CSL1, 2, and 3-that were isolated from chum salmon Oncorhynchus keta eggs on the respiratory burst activity in a macrophage cell line. The CSLs induced reactive oxygen species in the peritoneal macrophage cell line from rainbow trout Oncorhynchus mykiss (RTM5). However, this activity was not affected by l-rhamnose or dl-threo-1-phenyl-2-palmitoylamino-3-morpholino-1-propanol, an inhibitor for glucosylceramide synthesis, indicating that the interaction between CSLs and Gb3 on cell surfaces was not involved in the induction of respiratory burst activity.

    Original languageEnglish
    Pages (from-to)513-519
    Number of pages7
    JournalFisheries Science
    Volume79
    Issue number3
    DOIs
    Publication statusPublished - 2013 May 1

    Keywords

    • Lectin
    • Reactive oxygen species
    • Respiratory burst
    • Rhamnose-binding lectin

    ASJC Scopus subject areas

    • Aquatic Science

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