Rhamnose-binding lectins from steelhead trout (oncorhynchus mykiss) eggs recognize bacterial lipopolysaccharides and lipoteichoic acid

Hiroaki Tateno, Tomohisa Ogawa, Koji Muramoto, Hisao Kamiya, Mineo Saneyoshi

Research output: Contribution to journalArticlepeer-review

68 Citations (Scopus)

Abstract

The interaction between bacteria and three L-rhamnose-binding lectins, named STL1, STL2, and STL3, from steelhead trout (Oncorhynchus mykiss) eggs was investigated. Although STLs bound to most Gram-negative and Gram-positive bacteria, they agglutinated only Escherichia coli K-12 and Bacillus subtilis among the bacteria tested. The binding was inhibited by L-rhamnose. STLs bound to distinct serotypes of lipopolysaccharides (LPSs), and showed much higher binding activities to smooth-type LPSs of Escherichia coli K-12 and Shigella flexneri 1A than to their corresponding rough-type LPSs. STLs also bound to lipoteichoic acid (LTA) of Bacillus subtilis. These results indicate that STLs bound to bacteria by recognizing LPSs or LTA on the cell surfaces.

Original languageEnglish
Pages (from-to)604-612
Number of pages9
JournalBioscience, Biotechnology and Biochemistry
Volume66
Issue number3
DOIs
Publication statusPublished - 2002

Keywords

  • Animal lectin
  • Lipopolysaccharide
  • Lipoteichoic acid
  • Rhamnose-binding lectin
  • Steelhead trout

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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