Rhamnose-binding lectins from steelhead trout (oncorhynchus mykiss) eggs recognize bacterial lipopolysaccharides and lipoteichoic acid

Hiroaki Tateno, Tomohisa Ogawa, Koji Muramoto, Hisao Kamiya, Mineo Saneyoshi

    Research output: Contribution to journalArticlepeer-review

    70 Citations (Scopus)

    Abstract

    The interaction between bacteria and three L-rhamnose-binding lectins, named STL1, STL2, and STL3, from steelhead trout (Oncorhynchus mykiss) eggs was investigated. Although STLs bound to most Gram-negative and Gram-positive bacteria, they agglutinated only Escherichia coli K-12 and Bacillus subtilis among the bacteria tested. The binding was inhibited by L-rhamnose. STLs bound to distinct serotypes of lipopolysaccharides (LPSs), and showed much higher binding activities to smooth-type LPSs of Escherichia coli K-12 and Shigella flexneri 1A than to their corresponding rough-type LPSs. STLs also bound to lipoteichoic acid (LTA) of Bacillus subtilis. These results indicate that STLs bound to bacteria by recognizing LPSs or LTA on the cell surfaces.

    Original languageEnglish
    Pages (from-to)604-612
    Number of pages9
    JournalBioscience, Biotechnology and Biochemistry
    Volume66
    Issue number3
    DOIs
    Publication statusPublished - 2002

    Keywords

    • Animal lectin
    • Lipopolysaccharide
    • Lipoteichoic acid
    • Rhamnose-binding lectin
    • Steelhead trout

    ASJC Scopus subject areas

    • Biotechnology
    • Analytical Chemistry
    • Biochemistry
    • Applied Microbiology and Biotechnology
    • Molecular Biology
    • Organic Chemistry

    Fingerprint

    Dive into the research topics of 'Rhamnose-binding lectins from steelhead trout (oncorhynchus mykiss) eggs recognize bacterial lipopolysaccharides and lipoteichoic acid'. Together they form a unique fingerprint.

    Cite this