Abstract
The interaction between bacteria and three L-rhamnose-binding lectins, named STL1, STL2, and STL3, from steelhead trout (Oncorhynchus mykiss) eggs was investigated. Although STLs bound to most Gram-negative and Gram-positive bacteria, they agglutinated only Escherichia coli K-12 and Bacillus subtilis among the bacteria tested. The binding was inhibited by L-rhamnose. STLs bound to distinct serotypes of lipopolysaccharides (LPSs), and showed much higher binding activities to smooth-type LPSs of Escherichia coli K-12 and Shigella flexneri 1A than to their corresponding rough-type LPSs. STLs also bound to lipoteichoic acid (LTA) of Bacillus subtilis. These results indicate that STLs bound to bacteria by recognizing LPSs or LTA on the cell surfaces.
Original language | English |
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Pages (from-to) | 604-612 |
Number of pages | 9 |
Journal | Bioscience, Biotechnology and Biochemistry |
Volume | 66 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2002 |
Keywords
- Animal lectin
- Lipopolysaccharide
- Lipoteichoic acid
- Rhamnose-binding lectin
- Steelhead trout
ASJC Scopus subject areas
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry