Retrograde regulation of nuclear gene expression in CW-CMS of rice

Sota Fujii, Setsuko Komatsu, Kinya Toriyama

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The CW-cytoplasmic male sterility (CMS) line has the cytoplasm of Oryza rufipogon Griff, and mature pollen is morphologically normal under an optical microscope but lacks the ability to germinate; restorer gene Rf17 has been identified as restoring this ability. The difference between nuclear gene expression in mature anthers was compared for the CW-CMS line, [cms-CW] rf17rf17, and a maintainer line with normal cytoplasm of Oryza sativa L., [normal] rf17rf17. Using a 22-k rice oligoarray we detected 58 genes that were up-regulated more than threefold in the CW-CMS line. Expression in other organs was further investigated for 20 genes using RT-PCR. Five genes, including genes for alternative oxidase, were found to be preferentially expressed in [cms-CW] rf17rf17 but not in [normal] rf17rf17 or [cms-CW] Rf17Rf17. Such [cms-CW] rf17rf17-specific gene expression was only observed in mature anthers but not in leaves, stems, or roots, indicating the presence of anther-specific mitochondrial retrograde regulation of nuclear gene expression, and that Rf17 has a role in restoring the ectopic gene expression. We also used a proteomic approach to discover the retrograde regulated proteins and identified six proteins that were accumulated differently. These results reveal organ-specific induced mitochondrial retrograde pathways affecting nuclear gene expression possibly related to CMS.

Original languageEnglish
Pages (from-to)405-417
Number of pages13
JournalPlant Molecular Biology
Volume63
Issue number3
DOIs
Publication statusPublished - 2007 Feb 1

Keywords

  • Cytoplasmic male sterility
  • Oligoarray
  • Oryza rufipogon Griff
  • Oryza sativa L
  • Proteome
  • ms-CW

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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