Retinoid receptors in human breast carcinoma: Possible modulators of in situ estrogen metabolism

T. Suzuki, T. Moriya, A. Sugawara, N. Ariga, H. Takabayashi, H. Sasano

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Retinoid receptors (retinoic acid (RARs) and retinoid X (RXRs) receptors) were immunolocalized in 32 human invasive ductal breast carcinomas. These findings were correlated with clinicopathological parameters to study their biological significance in breast carcinoma. Retinoid receptor immunoreactivity, except for RXRγ, was detected in the nuclei of carcinoma cells. Percentage of positive cases were RARα; 81%, RARβ; 6%, RARγ; 28%, RXRα; 81%, and RXRβ; 59%. A significant correlation was detected between RARα labeling index (LI), and RXRα LI (r = 0.667, p < 0.001). Results from immunoblotting performed in three cases were consistent with those of immunohistochemistry. There was a significant correlation between RARα LI and 17β-hydroxysteroid dehydrogenase (17β-HSD) type 1 immunoreactivity (p < 0.05). A significant correlation was also detected between RARα (r = 0.413, p = 0.019) or RXRα (r = 0.429, p = 0.014) LI, and estrogen receptor (ER) LI. In T-47D breast cancer cells, which express RARα, RXRα and ER, 17β-HSD reductive activity increased 1.76-fold (p < 0.001), five days following treatment with 10 nM retinoic acid. These data suggest that retinoid receptors modulate various effects of retinoids, including estrogen metabolism in human breast carcinomas.

Original languageEnglish
Pages (from-to)31-40
Number of pages10
JournalBreast Cancer Research and Treatment
Volume65
Issue number1
DOIs
Publication statusPublished - 2001

Keywords

  • Breast carcinoma
  • Estrogen
  • Immunohistochemistry
  • RAR
  • RXR
  • Retinoids

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint

Dive into the research topics of 'Retinoid receptors in human breast carcinoma: Possible modulators of in situ estrogen metabolism'. Together they form a unique fingerprint.

Cite this