Retinoic acid is a potential negative regulator for differentiation of human periodontal ligament cells

Natsuko Shibuya, Eiji Nemoto, Sousuke Kanaya, Ryotaro Kunii, Hidetoshi Shimauchi

Research output: Contribution to journalReview article

Abstract

Retinoic acid (RA) exerts a wide variety of effects on development, cellular differentiation and homeostasis in various tissues. However, little is known about the effects of RA on the differentiation of periodontal ligament cells. In this study, we investigated whether RA can affect the dexamethasone-induced differentiation of periodontal ligament cells. Human periodontal ligament cells were differentiated via culturing in the presence of dexamethasone, ascorbic acid, and β-glycerophosphate for mineralized nodule formation, as characterized by von Kossa staining. Continuous treatment with all-trans-RA inhibited mineralization in a dose-dependent manner, with complete inhibition over 1 μM RA. Other RA analogs, 9-cis-RA and 13-cis-RA, were also effective. Furthermore, addition of RA for just the first 4 days completely inhibited mineralization; however, as RA was added at later stages of culture, the inhibitory effect was diminished, suggesting that RA induced a phase-dependent inhibition of mineralization. RA receptor (RAR)-α agonist (AM-580), but not retinoid X receptor agonist (methoprene acid), inhibited the mineralization, and RT-PCR analysis demonstrated that RAR-α was expressed on the cells, suggesting that RAR-α was involved in the inhibitory mechanism. This inhibition was accompanied by inhibition of alkaline phosphatase activity. These findings suggest that RA is a potential negative regulator for differentiation of human periodontal ligament cells.

Original languageEnglish
Pages (from-to)217-218
Number of pages2
JournalInternational Congress Series
Volume1284
DOIs
Publication statusPublished - 2005 Sep 1

Keywords

  • Differentiation
  • Human
  • Periodontal ligament cells
  • Retinoic acid

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint Dive into the research topics of 'Retinoic acid is a potential negative regulator for differentiation of human periodontal ligament cells'. Together they form a unique fingerprint.

  • Cite this