Retinoic acid inhibits elastase-induced injury in human lung epithelial cell lines

Mizue Nakajoh, Takeyasu Fukushima, Tomoko Suzuki, Mutsuo Yamaya, Katsutoshi Nakayama, Kiyohisa Sekizawa, Hidetada Sasaki

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The protective effects of retinoic acid on elastase-induced lung epithelial cell injury were studied using elastase extracted from purulent human sputum, the BEAS-2B human bronchial epithelial cell line, A549 human type II lung cell line, and primary cultures of human tracheal epithelial cells. Elastase decreased viability of BEAS-2B cells, A549 cells, and human tracheal epithelial cells in concentration- and time-dependent fashions. Elastase also induced apoptosis of BEAS-2B cells, A549 cells, and the tracheal epithelial cells detected with cell death detection enzyme-linked immunosorbent assay and terminal deoxyribonucleotidyl transferase-mediated dUTP-biotin nick-end labeling (TUNEL) methods. Retinoic acid alone did not affect the viability of BEAS-2B cells, A549 cells, or the tracheal epithelial cells, and did not induce apoptosis of the cells. However, retinoic acid prevented the decreases in the viability and reduced apoptosis of BEAS-2B cells, A549 cells, and the tracheal epithelial cells induced by elastase. Likewise, retinoic acid inhibited caspase 3 activity in BEAS-2B cells and A549 cells induced by elastase, as well as proteolytic activity of elastase. Furthermore, caspase 3 inhibitor inhibited the elastase-induced apoptosis of the cells. These findings suggest that retinoic acid may inhibit elastase-induced lung epithelial cell injury partly through the inhibition of proteolytic activity of elastase and through the inhibition of caspase 3 activity by elastase. Retinoic acid may, therefore, have protective effects against the elastase-induced lung injury and subsequent development of pulmonary emphysema.

Original languageEnglish
Pages (from-to)296-304
Number of pages9
JournalAmerican journal of respiratory cell and molecular biology
Volume28
Issue number3
DOIs
Publication statusPublished - 2003 Mar 1
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

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  • Cite this

    Nakajoh, M., Fukushima, T., Suzuki, T., Yamaya, M., Nakayama, K., Sekizawa, K., & Sasaki, H. (2003). Retinoic acid inhibits elastase-induced injury in human lung epithelial cell lines. American journal of respiratory cell and molecular biology, 28(3), 296-304. https://doi.org/10.1165/rcmb.4845