Retention behaviour of cardiac steroids using cyclodextrin in the mobile phase in high-performance liquid chromatography

Kazutake Shimada, Tomoyuki Oe, Yasuko Hirose, Yoshihiro Komine

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

The retention behaviour of twenty cardiac steroids and four fluorescent derivatives was examined by the addition of cyclodextrin to the mobile phase in reversed-phase high-performance liquid chromatography. The addition of a suitable cyclodextrin improved the separation of isomeric cardiac steroids. The steroid A B ring junction is the most important factor in the choice of the, optimum cyclodextrin to be added; the C D ring junction is less important. The hydroxyl group at the 3- or 12-position of the steroid enhanced the changes in retention times of these compounds. The effect of an unsaturated lactone ring at tie 17β-position on the retention in the presence of cyclodextrin was also determined with cardenolide (five-membered ring) and bufadienolide (six-membered ring) but little difference was observed. Isomeric cardiac steroids, whose separation has not been done by the conventional method, were clearly separated by this method. The flu orescence intensity of 3-(1-anthroyl)-cardiac steroid was enhanced by the addition of cyclodextrin to the mobile phase.

Original languageEnglish
Pages (from-to)339-347
Number of pages9
JournalJournal of Chromatography A
Volume478
Issue numberC
DOIs
Publication statusPublished - 1989
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Fingerprint

Dive into the research topics of 'Retention behaviour of cardiac steroids using cyclodextrin in the mobile phase in high-performance liquid chromatography'. Together they form a unique fingerprint.

Cite this