Restoration from polyglutamine toxicity after free electron laser irradiation of neuron-like cells

Miho Mohara, Takayasu Kawasaki, Ryuji Owada, Takayuki Imai, Hiroyasu Kanetaka, Shin ichi Izumi, Koichi Tsukiyama, Kazuhiro Nakamura

Research output: Contribution to journalArticle

Abstract

Proteins containing an expanded polyglutamine tract tend to aggregate, leading to the neuronal damage observed in polyglutamine diseases. We recently reported that free electron laser (FEL) irradiation markedly dissociates naked polyglutamine aggregates as well as the aggregate in the 293 T cells. In the present study, we investigated whether FEL irradiation of neuron-like cells with polyglutamine aggregates would restore the cellular damage and dysfunction. The aggregated polyglutamine peptides induced neurite retraction of differentiated SH-SY5Y cells. Upon FEL irradiation, the polyglutamine aggregates in the SH-SY5Y cells were dissociated, and the shorter length of individual neurite, fewer number of neurites per cell and shorter total length of neurite by polyglutamine were inhibited. Same results were essentially obtained in PC12 cells. Moreover, when FEL irradiation was applied to undifferentiated SH-SY5Y cells, the deficits in neuron-like differentiation seen in expanded polyglutamine peptide-containing cells were also rescued. Thus, FEL irradiation restored both the damage and differentiation caused by polyglutamine in neuron-like cells.

Original languageEnglish
Pages (from-to)42-49
Number of pages8
JournalNeuroscience Letters
Volume685
DOIs
Publication statusPublished - 2018 Oct 15

Keywords

  • Free electron laser
  • Neurite
  • Polyglutamine disease

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Restoration from polyglutamine toxicity after free electron laser irradiation of neuron-like cells'. Together they form a unique fingerprint.

  • Cite this