Respiration activity of Escherichia coli entrapped in a cone-shaped microwell and cylindrical micropore monitored by scanning electrochemical microscopy (SECM)

Takatoshi Kaya; Daisuke Numai; Kuniaki Nagamine; Shigeo Aoyagi; Hitoshi Shiku; Tomokazu Matsue

doi:10.1039/b316582e

Respiration activity of Escherichia coli entrapped in a cone-shaped microwell and cylindrical micropore monitored by scanning electrochemical microscopy (SECM)

Takatoshi Kaya, Daisuke Numai, Kuniaki Nagamine, Shigeo Aoyagi, Hitoshi Shiku, Tomokazu Matsue

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

The metabolic activity of E. coli cells embedded in collagen gel microstructures in a cone-shaped well and in a cylindrical micropore was investigated using scanning electrochemical microscopy (SECM), based on the oxygen consumption rate and the conversion rate from ferrocyanide to ferricyanide. The analysis of the concentration profiles for oxygen and ferrocyanide afforded the oxygen consumption rate and the ferrocyanide production rate. A comparison indicated that the ferrocyanide production rates were larger than the oxygen consumption rate, and also that the rates observed in the cylindrical micropore were larger than those observed in the cone-shaped well. The ferrocyanide production rate of a single E. coli cell was calculated to be (5.4 ± 2.6) × 10^-19 mol s^-1, using a cylindrical micropore system.

Original language	English
Pages (from-to)	529-534
Number of pages	6
Journal	Analyst
Volume	129
Issue number	6
DOIs	https://doi.org/10.1039/b316582e
Publication status	Published - 2004 Jun

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry

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10.1039/b316582e

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abstract = "The metabolic activity of E. coli cells embedded in collagen gel microstructures in a cone-shaped well and in a cylindrical micropore was investigated using scanning electrochemical microscopy (SECM), based on the oxygen consumption rate and the conversion rate from ferrocyanide to ferricyanide. The analysis of the concentration profiles for oxygen and ferrocyanide afforded the oxygen consumption rate and the ferrocyanide production rate. A comparison indicated that the ferrocyanide production rates were larger than the oxygen consumption rate, and also that the rates observed in the cylindrical micropore were larger than those observed in the cone-shaped well. The ferrocyanide production rate of a single E. coli cell was calculated to be (5.4 ± 2.6) × 10-19 mol s-1, using a cylindrical micropore system.",

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T1 - Respiration activity of Escherichia coli entrapped in a cone-shaped microwell and cylindrical micropore monitored by scanning electrochemical microscopy (SECM)

AU - Kaya, Takatoshi

AU - Numai, Daisuke

AU - Nagamine, Kuniaki

AU - Aoyagi, Shigeo

AU - Shiku, Hitoshi

AU - Matsue, Tomokazu

PY - 2004/6

Y1 - 2004/6

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AB - The metabolic activity of E. coli cells embedded in collagen gel microstructures in a cone-shaped well and in a cylindrical micropore was investigated using scanning electrochemical microscopy (SECM), based on the oxygen consumption rate and the conversion rate from ferrocyanide to ferricyanide. The analysis of the concentration profiles for oxygen and ferrocyanide afforded the oxygen consumption rate and the ferrocyanide production rate. A comparison indicated that the ferrocyanide production rates were larger than the oxygen consumption rate, and also that the rates observed in the cylindrical micropore were larger than those observed in the cone-shaped well. The ferrocyanide production rate of a single E. coli cell was calculated to be (5.4 ± 2.6) × 10-19 mol s-1, using a cylindrical micropore system.

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Respiration activity of Escherichia coli entrapped in a cone-shaped microwell and cylindrical micropore monitored by scanning electrochemical microscopy (SECM)

Abstract

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