Respiration activity of Escherichia coli entrapped in a cone-shaped microwell and cylindrical micropore monitored by scanning electrochemical microscopy (SECM)

Takatoshi Kaya, Daisuke Numai, Kuniaki Nagamine, Shigeo Aoyagi, Hitoshi Shiku, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

The metabolic activity of E. coli cells embedded in collagen gel microstructures in a cone-shaped well and in a cylindrical micropore was investigated using scanning electrochemical microscopy (SECM), based on the oxygen consumption rate and the conversion rate from ferrocyanide to ferricyanide. The analysis of the concentration profiles for oxygen and ferrocyanide afforded the oxygen consumption rate and the ferrocyanide production rate. A comparison indicated that the ferrocyanide production rates were larger than the oxygen consumption rate, and also that the rates observed in the cylindrical micropore were larger than those observed in the cone-shaped well. The ferrocyanide production rate of a single E. coli cell was calculated to be (5.4 ± 2.6) × 10-19 mol s-1, using a cylindrical micropore system.

Original languageEnglish
Pages (from-to)529-534
Number of pages6
JournalAnalyst
Volume129
Issue number6
DOIs
Publication statusPublished - 2004 Jun

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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