Respiration accumulates Calvin cycle intermediates for the rapid start of photosynthesis in Synechocystis sp. PCC 6803

Ginga Shimakawa, Tomohisa Hasunuma, Akihiko Kondo, Mami Matsuda, Amane Makino, Chikahiro Miyake

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

We tested the hypothesis that inducing photosynthesis in cyanobacteria requires respiration. A mutant deficient in glycogen phosphorylase (ΔGlgP) was prepared in Synechocystis sp. PCC 6803 to suppress respiration. The accumulated glycogen in ΔGlgP was 250-450% of that accumulated in wild type (WT). The rate of dark respiration in ΔGlgP was 25% of that inWT. In the dark, P700+ reduction was suppressed in ΔGlgP, and the rate corresponded to that in (2,5-dibromo-3-methyl-6-isopropyl- p -benzoquinone)-treated WT, supporting a lower respiration rate in ΔGlgP. Photosynthetic O2-evolution rate reached a steady-state value much slower in ΔGlgP than in WT. This retardation was solved by addition of D-glucose. Furthermore, we found that the contents of Calvin cycle intermediates in ΔGlgP were lower than those in WT under dark conditions. These observations indicated that respiration provided the carbon source for regeneration of ribulose 1,5-bisphosphate in order to drive the rapid start of photosynthesis.

Original languageEnglish
Pages (from-to)1997-2007
Number of pages11
JournalBioscience, Biotechnology and Biochemistry
Volume78
Issue number12
DOIs
Publication statusPublished - 2014

Keywords

  • Cyanobacteria
  • Glycogen phosphorylase
  • Photosynthesis
  • Respiration

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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